Photo-reactive oligodeoxynucleotide-embedded nanovesicles (PROsomes) with switchable stability for efficient cellular uptake and gene knockdown.

Chemical Communications : Chem Comm
Beob Soo KimKanjiro Miyata

Abstract

A photo-responsive nanovesicle is fabricated by polyion complex (PIC) formation between poly(ethylene glycol) (PEG)-block-polypeptides and photo-reactive oligodeoxynucleotides (PROs)/anti-sense oligonucleotides (ASOs). The ultraviolet (UV) light triggers reversible crosslinking between PROs and ASOs in the vesicular membrane, providing the nanovesicle with switchable stability under physiological conditions. The resulting nanovesicle allows efficient cellular internalization, leading to significant UV-triggered gene knockdown in cultured cells.

References

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Citations

May 7, 2021·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Akihiro Kishimura

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Methods Mentioned

BETA
gene knockdown
size exclusion chromatography
dynamic
transmission electron microscopy
static light scattering
fluorescence correlation spectroscopy
FCS
flow cytometry

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