Jan 7, 2009

Photoacoustic monitoring of burn healing process in rats

Journal of Biomedical Optics
Kazuya AizawaMinoru Obara


We performed multiwavelength photoacoustic (PA) measurement for extensive deep dermal burns in rats to monitor the healing process of the wounds. The PA signal peak at 532 nm, an isosbestic point for oxyhemoglobin (HbO(2)) and deoxyhemoglobin (HHb), was found to shift to a shallower region of the injured skin tissue with the elapse of time. The results of histological analysis showed that the shift of the PA signal reflected angiogenesis in the wounds. Until 24 h postburn, PA signal amplitude generally increased at all wavelengths. We speculate that this increase in amplitude is associated with dilation of blood vessels within healthy tissue under the injured tissue layer and increased hematocrit value due to development of edema. From 24 to 48 h postburn, the PA signal showed wavelength-dependent behaviors; signal amplitudes at 532, 556, and 576 nm continued to increase, while amplitude at 600 nm, an HHb absorption-dominant wavelength, decreased. This seems to reflect change from shock phase to hyperdynamic state in the rat; in the hyperdynamic state, cardiac output and oxygen consumption increased considerably. These findings show that multiwavelength PA measurement would be useful for monitoring recovery of perfusion and cha...Continue Reading

  • References29
  • Citations8


  • References29
  • Citations8


Mentioned in this Paper

Skin Tissue
Angiogenic Process
Blood Vessel
Pathological Dilatation
Tumor Angiogenesis
Oxyhemoglobin Measurement
Photoacoustic Spectroscopy
Acoustic Radiation Force Impulse Imaging

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