Photoacoustic shadow-casting microscopy

Optics Letters
Jorge Tordera MoraLiang Gao

Abstract

We present photoacoustic shadow-casting microscopy (PASM), a technique that allows high-resolution imaging of weakly absorbing biological samples with unprecedented sensitivity. In PASM, a uniform optical absorbing layer is placed in contact with the samples and is excited by the light transmitted through the sample, producing photoacoustic (PA) waves with an increased signal-to-noise ratio compared with that generated by the sample itself. Therefore, given a desired image quality, the required excitation fluence is much reduced, alleviating the photothermal damage to the specimen. The system provides a lateral resolution of 5 μm when using a 0.30 NA microscope objective lens. To demonstrate PASM, we present images of bovine red blood cells and microbeads.

References

Feb 5, 2013·Journal of Biomedical Optics·Liang GaoLihong V Wang
Sep 13, 2013·Journal of Biomedical Optics·Amy M WinklerLihong V Wang
Jun 7, 2014·Annual Review of Biomedical Engineering·Lihong V Wang, Liang Gao
Aug 12, 2014·Journal of Biomedical Optics·Amos DanielliLihong V Wang
Oct 11, 2014·Photoacoustics·Junjie Yao, Lihong V Wang
Jul 29, 2016·Nature Methods·Judith WeberSarah E Bohndiek
Oct 14, 2017·Journal of the American Chemical Society·Jingjing ZhangYi Lu
Nov 22, 2015·Photoacoustics·Sung-Liang ChenXueding Wang

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