Photoactivatable aggregation-induced emission probes for lipid droplets-specific live cell imaging

Chemical Science
Meng GaoBen Zhong Tang

Abstract

Photoactivatable probes for lipid droplets (LDs)-specific live-cell imaging are powerful tools for investigating their biological functions through precise spatial and temporal control. Ideal photoactivatable probes for LDs imaging require high concentration accumulation of fluorophores in LDs, simple synthetic procedures, and excellent photoactivation efficiency. However, it is difficult to overcome these challenges by conventional fluorophores due to aggregation-caused quenching (ACQ). In this study, a new class of photoactivatable and LDs-specific fluorescent probes was developed based on dihydro-2-azafluorenones, which can easily undergo photooxidative dehydrogenation reaction to afford 2-azafluorenones with aggregation-induced emission (AIE) properties. Dihydro-2-azafluorenones as photoactivatable and LDs-specific probes display significant advantages of excellent photoactivation efficiency and lack of self-quenching in the aggregated state, and are expected to have broad applications in study of biological functions of LDs' through light-controlled spatiotemporal imaging.

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Citations

Aug 25, 2017·Chemical Communications : Chem Comm·Zhenglin ZhangOgnjen Š Miljanić
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Feb 1, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiujuan ShiBen Zhong Tang
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Jul 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lei ShiXiao-Qi Yu
Apr 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yaqi YangBen Zhong Tang
Oct 8, 2019·The Journal of Organic Chemistry·Sheng XieMingdi Yan
Nov 16, 2017·ACS Applied Materials & Interfaces·Ju MeiHe Tian
Aug 23, 2019·Accounts of Chemical Research·Dong Wang, Ben Zhong Tang
Feb 11, 2020·Analytical Chemistry·Xin ZhangZhangjun Hu
Nov 12, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Weihua ZhuangYunbing Wang

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Methods Mentioned

BETA
column chromatography
NMR
X-ray
Fluorescence

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