PMID: 8946384Nov 25, 1996Paper

Photoaffinity labeling of mefloquine-binding proteins in human serum, uninfected erythrocytes and Plasmodium falciparum-infected erythrocytes

Molecular and Biochemical Parasitology
J DesnevesL Tilley

Abstract

A photoreactive quinolinemethanol analog, N-[4-[1-hydroxy-2-(dibutylamino)ethyl]quinolin-8yl]-4- azido-2-salicylamide (ASA-MQ) has been synthesized which closely mimics the action of mefloquine. ASA-MQ possesses potent antimalarial activity against a mefloquine-sensitive strain of Plasmodium falciparum and shows decreased activity against a mefloquine-resistant parasite strain. Radioiodinated ASA-MQ has been used in photoaffinity labeling studies to identify mefloquine-interacting proteins in serum, uninfected erythrocytes and Plasmodium falciparum-infected erythrocytes. We have shown that mefloquine interacts specifically with apo-A1, the major protein of serum high density lipoproteins. In addition, mefloquine was shown to interact specifically with the erythrocyte membrane protein, band 7.2b (stomatin). A further two high affinity mefloquine-binding proteins with apparent molecular masses of 22 and 36 kDa were identified in three different strains of Plasmodium falciparum. We suggest that these two mefloquine-binding parasite proteins may be involved in the uptake of mefloquine or may represent macromolecular targets of mefloquine action in malaria parasites.

References

Jun 1, 1978·Antimicrobial Agents and Chemotherapy·L H SchmidtD Vaughan
Feb 1, 1979·Antimicrobial Agents and Chemotherapy·C D FitchR Chevli
Aug 20, 1976·Science·W Trager, J B Jensen
Dec 1, 1992·The Journal of Infectious Diseases·F O ter KuileN J White
Aug 27, 1991·Biochimica Et Biophysica Acta·C M Hiebl-DirschmiedR Prohaska
Oct 1, 1988·Biochemical Pharmacology·G VanderkooiY Yuthavong
Jun 1, 1986·Annals of Tropical Medicine and Parasitology·T G GearyH Ginsburg
Jul 1, 1974·The Journal of Clinical Investigation·C D FitchY Gonzalez
Nov 1, 1968·Journal of Medicinal Chemistry·W G DuncanD W Henry
Mar 23, 1984·Biochimica Et Biophysica Acta·R C San GeorgeM E Fabry
May 1, 1995·British Journal of Clinical Pharmacology·B CenniB Betschart
Oct 1, 1994·Molecular and Biochemical Parasitology·A BermanL Tilley
Mar 1, 1994·Transactions of the Royal Society of Tropical Medicine and Hygiene·C LuxemburgerN J White
Apr 1, 1993·Molecular and Biochemical Parasitology·S R KarczA F Cowman
Jan 29, 1994·BMJ : British Medical Journal·N J White
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·A F CowmanJ K Thompson
Aug 31, 1993·Biochemical and Biophysical Research Communications·A C Chou, C D Fitch
Jul 5, 1996·Biochemical and Biophysical Research Communications·J DesnevesL Tilley
Feb 1, 1947·Journal of the American Chemical Society·E R BUCHMANJ A SENEKER
Sep 1, 1946·Journal of the American Chemical Society·R E LUTZ, P S BAILEY

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Citations

Feb 1, 1997·International Journal for Parasitology·M Foley, L Tilley
Aug 27, 1998·Pharmacology & Therapeutics·M Foley, L Tilley
Aug 8, 2002·Molecular and Biochemical Parasitology·Akinola AdisaLeann Tilley
Aug 31, 2001·Biochemical and Biophysical Research Communications·H J SchäferG Grüber
Aug 26, 1998·Experimental Parasitology·L M Chow, S K Volkman
Oct 15, 2013·The Journal of Pharmacy and Pharmacology·Lissinda H du PlessisAwie F Kotzé
Dec 18, 2019·Antimicrobial Agents and Chemotherapy·José Luiz Fernandes VieiraMichelle Valeria Dias Ferreira
Aug 3, 1999·Antimicrobial Agents and Chemotherapy·L E BermudezL S Young

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