PMID: 3768420Nov 19, 1986Paper

Photoaffinity labeling of thaumatin-binding protein in monkey circumvallate papillae

Biochimica Et Biophysica Acta
K ShimazakiM Nakao

Abstract

Thaumatin I is an intensely sweet-tasting protein. It was photo-crosslinked with taste papillae of crab-eating monkey by using a conjugated photo-affinity reagent [3H]azidobenzoylthaumatin I. Serial sections of SDS-polyacrylamide gel electrophoresis of the 0.1 M sodium phosphate buffer-soluble fraction from taste papillae had a large peak of radioactivity at the Mr region of approx. 70,000; fractions from non-taste papillae did not. Excess unlabeled thaumatin I reduced the photo-crosslinking at the 70 kDa region; acetylated thaumatin I (which is not sweet) did not. The results show that taste papillae of the monkey contain a protein of Mr approx. 50,000, which binds to thaumatin I (Mr 22,209) but not to completely acetylated thaumatin I. The possibility that the thaumatin-binding protein is a sweet receptor protein is discussed.

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R H Cagan, R W Morris
Jan 1, 1978·Folia primatologica; international journal of primatology·D GlaserH van der Wel
May 2, 1979·European Journal of Biochemistry·R B IyengarP D van Wassenaar
Jul 1, 1971·Comparative Biochemistry and Physiology. A, Comparative Physiology·D S Smith, J R Linton
Jun 15, 1971·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Y HijiM Sato
Nov 5, 1981·Biochimica Et Biophysica Acta·K ShimazakiT Takegami

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Citations

Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·D MingR F Margolskee
Jan 30, 1990·Biochemical and Biophysical Research Communications·R S Hosmane, C M Bertha
Dec 5, 1992·Journal of Molecular Biology·C M OgataS H Kim
Jan 1, 1992·Critical Reviews in Food Science and Nutrition·Y Kurihara
Jul 1, 1996·Biopolymers·H IzawaY Ariyoshi

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