PMID: 20650119Aug 1, 1995Paper

Photobinding of drugs to cells as an indicator of potential photoallergy

Toxicology in Vitro : an International Journal Published in Association with BIBRA
M A MirandaL A Martinez

Abstract

The photobinding of drugs to cells has been investigated as a possible indicator of the photoallergic potential. Tiaprofenic acid (TA), carprofen (CP), benoxaprofen (BP) and ibuprofen (IP) were selected as test substances and human fibroblasts as model biological system. Radioactive labelling of the drugs was achieved through alkaline exchange of the alpha-carboxyl hydrogens by tritium, using (3)H(2)O as solvent. When the labelled compounds were co-irradiated with fibroblasts, TA gave rise to the highest amount of radioactivity covalently bound to cells. This also occurred, albeit to a lower extent, with CP and BP; by contrast, no effect was observed for IP. The observed rank order is in agreement with the available in vivo data. The time course of the process was determined for TA, distinguishing between covalent and non-covalent binding. The results, together with gas Chromatographie analyses of the irradiation mixtures and binding studies with TA and its major photoproduct decarboxytiaprofenic acid (decarboxy-TA) in the dark, indicated that most of the observed photobinding might be due to the photoproduct rather than to the parent drug. It is conceivable that hydrogen abstraction by the excited ketone could take place with ...Continue Reading

References

Aug 1, 1992·Photochemistry and Photobiology·A AsthanaR W Tuveson
Jul 1, 1991·Journal of the American Academy of Dermatology·E HölzleG Plewig
Jul 1, 1985·Drugs·J H Epstein, B U Wintroub
Jan 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·W W Lovell

❮ Previous
Next ❯

Citations

Oct 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M A MirandaM J Gómez-Lechón
Aug 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M A MirandaJ V Castell
Feb 24, 2001·Journal of Photochemistry and Photobiology. B, Biology·J MoserA Hye
Jun 26, 1998·Journal of Photochemistry and Photobiology. B, Biology·F Boscá, M A Miranda
Dec 30, 1998·Journal of Photochemistry and Photobiology. B, Biology·L A MartínezE Oliveros
Oct 24, 1997·Photochemistry and Photobiology·H de VriesG M Beijersbergen an Henegouwen
Feb 8, 2007·Chembiochem : a European Journal of Chemical Biology·Jérôme TrzcionkaMiguel Angel Miranda

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.