Photobiomodulation combined with photodynamic therapy using ruthenium phthalocyanine complexes in A375 melanoma cells: Effects of nitric oxide generation and ATP production

Journal of Photochemistry and Photobiology. B, Biology
Laísa Bonafim NegriMichael R Hamblin

Abstract

Light irradiation has been used in clinical therapy for several decades. In this context, photobiomodulation (PBM) modulates signaling pathways via ROS, ATP, Ca2+, while photodynamic therapy (PDT) generates reactive oxygen species by excitation of a photosensitizer. NO generation could be an important tool when combined with both kinds of light therapy. By using a metal-based compound, we found that PBM combined with PDT could be a beneficial cancer treatment option. We used two types of ruthenium compounds, ([Ru(Pc)], Pc = phthalocyanine) and trans-[Ru(NO)(NO2)(Pc)]. The UV-vis spectra of both complexes displayed a band in the 660 nm region. In the case of 0.5 μM trans-[Ru(NO)(NO2)(Pc)], light irradiation at the Q-band reduced the percentage of viable human melanoma (A375) cells to around 50% as compared to [Ru(Pc)]. We hypothesized that these results were due to a synergistic effect between singlet oxygen and nitric oxide. Similar experiments performed with PDT (660 nm) combined with PBM (850 nm) induced more photocytotoxicity using both [Ru(Pc)] and trans-[Ru(NO)(NO2)(Pc)]. This was interpreted as PBM increasing cell metabolism (ATP production) and the consequent higher uptake of the ruthenium phthalocyanine compounds and mo...Continue Reading

References

Apr 1, 1974·Journal of the National Cancer Institute·T J Dougherty
Jun 24, 1998·Journal of the National Cancer Institute·T J DoughertyQ Peng
Oct 5, 2001·Expert Opinion on Pharmacotherapy·C H SibataT J Kinsella
Mar 29, 2007·International Journal of Cancer. Journal International Du Cancer·Yiyao LiuMichihiro Nakamura
Feb 27, 2008·Journal of Medicinal Chemistry·Frédéric SchmittBruno Therrien
May 13, 2008·Nitric Oxide : Biology and Chemistry·Lisa A RidnourDavid A Wink
Jul 25, 2008·Photochemistry and Photobiology·Tiina I Karu
Aug 7, 2008·Dalton Transactions : an International Journal of Inorganic Chemistry·Zênis Novais da RochaRoberto Santana da Silva
Aug 30, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Mateusz KwitniewskiJohan Moan
Sep 3, 2009·Journal of the American Chemical Society·M Salomé Rodríguez-MorgadeTomás Torres
Nov 17, 2009·Methods in Enzymology·Naoto Oku, Takayuki Ishii
Feb 5, 2010·The Journal of Investigative Dermatology·Christian CallesPeter Schroeder
Mar 12, 2010·Apoptosis : an International Journal on Programmed Cell Death·Abhishek D GargPatrizia Agostinis
Jun 18, 2010·Chemical Society Reviews·Salvatore Sortino
Oct 1, 2008·Coordination Chemistry Reviews·Michael J Rose, Pradip K Mascharak
Mar 15, 2012·Nature Communications·Mikhail G ShapiroFrancisco Bezanilla
Jun 29, 2013·Biochemical Pharmacology·Julio C Rojas, F Gonzalez-Lima
Jan 24, 2014·Dalton Transactions : an International Journal of Inorganic Chemistry·Tassiele A HeinrichRoberto Santana da Silva
Mar 5, 2014·The British Journal of Radiology·S R McKeown
Apr 22, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Aaron C Anselmo, Samir Mitragotri
Dec 1, 2004·Photodiagnosis and Photodynamic Therapy·Ana P CastanoMichael R Hamblin
Dec 3, 2014·Photodiagnosis and Photodynamic Therapy·Shang-Ru TsaiMichael R Hamblin
Apr 10, 2015·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Bryan Q SpringTayyaba Hasan
Jun 13, 2015·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Moumita GangopadhyayN D Pradeep Singh
Nov 8, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jan Willem KleinovinkFerry Ossendorp
Mar 5, 2017·Photodiagnosis and Photodynamic Therapy·Catherine ChristieHenry Hirschberg
Apr 26, 2017·Journal of Photochemistry and Photobiology. B, Biology·Shang-Ru Tsai, Michael R Hamblin
Apr 11, 2018·Antioxidants & Redox Signaling·Veena SomasundaramDavid A Wink
Jun 9, 2018·Journal of Biomedical Nanotechnology·Natalia Maria CandidoMarília Freitas Calmon
Aug 25, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Aurore Fraix, Salvatore Sortino

❮ Previous
Next ❯

Citations

Nov 23, 2020·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Christelle FayadCostantine F Daher
Mar 7, 2021·Journal of Photochemistry and Photobiology. B, Biology·Clara M G de FariaVanderlei S Bagnato
Jun 29, 2021·Photodermatology, Photoimmunology & Photomedicine·Zeqing ChenMuqing Liu
Aug 7, 2021·International Journal of Biological Macromolecules·Farnaz AzadikhahMahnaz Hadizadeh
Oct 5, 2021·Frontiers in Bioengineering and Biotechnology·Letícia B SilvaPriscyla D Marcato

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis