Photobiomodulation with 808-nm diode laser light promotes wound healing of human endothelial cells through increased reactive oxygen species production stimulating mitochondrial oxidative phosphorylation

Lasers in Medical Science
Andrea AmaroliLaura Vergani

Abstract

Photobiomodulation of cells using near-infrared (NIR) monochromatic light can affect cell functions such as proliferation, viability, and metabolism in a range of cell types. Evidence for the effects of near-infrared light on endothelial cells has been reported, but the studies were mainly performed using VIS light emitted by low-energy lasers, because NIR wavelengths seemed negatively stimulate these cells. Cell viability, free radical-induced oxidative stress, NF-κB activation, nitric oxide release, mitochondrial respiration, and wound healing repair were assessed in human endothelial cells (HECV) irradiated with 808-nm diode laser light (laser setup = 1 W/cm2, 60 s, 60 J/cm2, CW vs measured energy parameter = 0.95 W/cm2, 60 s, 57 J/cm2, mode CW) emitted by an handpiece with flat-top profile. No difference in viability was detected between controls and HECV cells irradiated with 808-nm diode laser light for 60 s. Irradiated cells demonstrated higher proliferation rate and increased migration ability associated to moderate increase in ROS production without a significant increase in oxidative stress and oxidative stress-activated processes. Near-infrared light stimulated mitochondrial oxygen consumption and ATP synthesis in HE...Continue Reading

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Citations

Apr 30, 2019·Journal of Biophotonics·Silvia RaveraAndrea Amaroli
Jun 5, 2020·Lasers in Surgery and Medicine·Andrew McCollochMichael Cho
Jul 2, 2020·Dentistry Journal·Steven ParkerMartin Grootveld
Mar 21, 2020·Lasers in Medical Science·Mohamed Salaheldien Mohamed AlayatAmir Abdel-Raouf El-Fiky
Jun 11, 2020·Case Reports in Medicine·Elisabetta MerigoCarlo Fornaini
Sep 12, 2020·International Journal of Molecular Sciences·Laura Marinela Ailioaie, Gerhard Litscher
Feb 23, 2020·Journal of Clinical Medicine·Letizia FerroniBarbara Zavan
Dec 2, 2020·International Journal of Molecular Sciences·Yujin OhsugiTakanori Iwata
Jan 3, 2021·Lasers in Medical Science·Adenilson de Souza da FonsecaAndre Luiz Mencalha
Apr 20, 2021·Cell Biochemistry and Function·Olajumoke OyebodeHeidi Abrahamse
Jul 6, 2021·Journal of Physical Therapy Science·Mohamed Salaheldien Mohamed AlayatEhab Mohamed Abdel-Kafy
Nov 28, 2021·Journal of Biophotonics·Caleb LiebmanMichael Cho

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