Photochemical cross-linking of 125I-hydroxyphenylisopropyl adenosine to the A1 adenosine receptor of rat adipocytes

FEBS Letters
A GreenM Partin

Abstract

Rat adipocyte plasma membranes were incubated with the A1 adenosine receptor agonist, 125I-hydroxyphenylisopropyl adenosine (1 nM) and then treated with the photoactive cross-linking agent, ANB-NOS. The membranes were solubilized and analyzed by SDS-PAGE and autoradiography. A single protein, Mr approx. 38,000, was specifically labeled. Reduction with 2-mercaptoethanol did not affect the apparent Mr of the labeled protein. Labeling was inhibited by the adenosine receptor agonists, HPIA, PIA and NECA, and by the antagonist, theophylline, but was unaffected by inosine. We conclude that the A1 adenosine receptor is a single protein of Mr approx. 38,000.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C Londos, J Wolff
Aug 30, 1985·Biochemical and Biophysical Research Communications·J I ChocaR D Green
Oct 1, 1982·Naunyn-Schmiedeberg's Archives of Pharmacology·U SchwabeH Glossmann
Oct 1, 1982·Canadian Journal of Physiology and Pharmacology·R Munshi, H P Baer

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Citations

Aug 14, 2019·The American Journal of Medicine·Elizabeth MostofskySuzanne M Bertisch
Aug 30, 1989·Biochemical and Biophysical Research Communications·A GreenM Partin

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