PMID: 9177190Jun 10, 1997Paper

Photochemical electron injection into redox-active proteins

Proceedings of the National Academy of Sciences of the United States of America
Peter Brzezinski, M T Wilson

Abstract

A new method is presented that makes it possible to inject electrons rapidly into redox-active proteins by means of a short light flash. Reduced carboxymethylated cytochrome c (CmCyt c) with carbon monoxide bound to the heme iron is mixed with the oxidized acceptor protein. Upon rapid photodissociation of CO the apparent redox potential of CmCyt c drops, resulting in electron transfer to the electron acceptor. In this study we have used mitochondrial cytochrome c oxidase as the acceptor protein, but the method also can be used to investigate electron transfer to other proteins that can interact with cytochrome c. In principle, it can be used with any redox protein into which a CO binding site at the heme iron can be engineered.

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Citations

Jul 16, 2003·Journal of Molecular Biology·Stefano GianniHeinrich Roder
Dec 24, 2002·Biochimica Et Biophysica Acta·Randy W Larsen
Feb 15, 2000·European Journal of Biochemistry·O FarverI Pecht
Sep 6, 2000·European Journal of Biochemistry·A KotlyarO Einarsdóttir
Oct 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Martin TrouillardFabrice Rappaport
Sep 8, 2011·Photochemistry and Photobiology·Julien Bonin, Marc Robert
Dec 21, 2010·Biochemistry. Biokhimii︠a︡·M S KritskyO A Golub
Apr 4, 2009·Doklady. Biochemistry and Biophysics·M S KritskyV O Popov
Nov 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Eric PiletMarten H Vos
Sep 10, 2008·Biochimica Et Biophysica Acta·Małgorzata BrindellRudi van Eldik
Apr 22, 2004·Biochimica Et Biophysica Acta·Olöf Einarsdóttir, Istvan Szundi
Nov 13, 2007·Biochimica Et Biophysica Acta·Marten H Vos
Jul 20, 2016·Proceedings of the National Academy of Sciences of the United States of America·Camilla Rydström LundinPeter Brzezinski
Feb 22, 2017·Physical Chemistry Chemical Physics : PCCP·Marten H VosUrsula Liebl
Jun 1, 2001·Methods : a Companion to Methods in Enzymology·A BellelliM T Wilson
Jun 19, 1998·Biochimica Et Biophysica Acta·M KarpeforsP Brzezinski
Sep 18, 2012·The Journal of Physical Chemistry. B·Karin NienhausG Ulrich Nienhaus

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