PMID: 16512410Mar 4, 2006Paper

Photochemical fishing approaches for identifying target proteins and elucidating the structure of a ligand-binding region using carbene-generating photoreactive probes

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Yutaka Sadakane, Yasumaru Hatanaka

Abstract

Photoaffinity labeling enables the direct probing of a target protein through a covalent bond between a ligand and its binding protein, and even a complex formed by weak interactions can be isolated by the method. The photochemical fishing approach accelerates the throughput, isolating crosslinked complexes and analyzing the structure of the ligand binding site within the protein. We used carbene-generating phenyldiazirine for this approach because practical examinations had shown that the phenyldiazirine functioned as the powerful barb on the hook. Improving the synthetic pathways of the photoprobes and using chemoselective-integrated photoreactive units makes possible the easy and rapid preparation of carbene-generating photoreactive probes including the derivatives in peptides, proteins, DNAs, and carbohydrates. This review also shows several recent impacts of photoaffinity labeling, including the in vivo preparation of photoreactive proteins in living cells.

References

Jan 1, 1977·Methods in Enzymology·G L Kenyon, T W Bruice
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P S PendergrastR H Ebright
Jan 1, 1985·Analytical Biochemistry·L B Shih, H Bayley
Jul 25, 1973·Journal of the American Chemical Society·R A Smith, J R Knowles
Jan 1, 1971·Helvetica Chimica Acta·R Schwyzer, M Caviezel
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·S W Yang, H A Nash
May 17, 1994·Biochemistry·G Dormán, G D Prestwich
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·M A ClearyW Herr
Jan 9, 1999·Trends in Biotechnology·G A Lemieux, C R Bertozzi
Feb 18, 1999·Current Opinion in Chemical Biology·B L Drees
Dec 2, 1999·Methods : a Companion to Methods in Enzymology·S M SenguptaC L Peterson
Feb 1, 2000·Trends in Biotechnology·G Dormán, G D Prestwich
Jun 24, 2000·Nature·A Pandey, M Mann
Aug 19, 2000·Nature·J C SamuelsonR E Dalbey
Aug 24, 2000·The Journal of Organic Chemistry·K A Chehade, H P Spielmann
Sep 2, 2000·The Journal of Organic Chemistry·Y HatanakaP Jong-Jip
Nov 1, 2000·Nucleic Acids Research·M Zofall, B Bartholomew
Apr 17, 2001·The Journal of Biological Chemistry·S M SenguptaB Bartholomew
Apr 21, 2001·Science·L WangP G Schultz
May 25, 2001·Nature Structural Biology·A J DriessenC van der Does
Apr 12, 2002·Current Topics in Medicinal Chemistry·Yasumaru Hatanaka, Yutaka Sadakane
Aug 1, 2002·Journal of the American Chemical Society·Jason W ChinPeter G Schultz
Aug 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jason W ChinPeter G Schultz
Dec 10, 2002·Current Opinion in Chemical Biology·Takahiro Hohsaka, Masahiko Sisido
Jan 28, 2003·Current Opinion in Chemical Biology·Heng Zhu, Michael Snyder

❮ Previous
Next ❯

Citations

Nov 7, 2007·Journal of the American Chemical Society·Andrew L MacKinnonJack Taunton
Oct 23, 2012·The Plant Journal : for Cell and Molecular Biology·Miaoying TianDaniel F Klessig
Feb 17, 2015·Future Medicinal Chemistry·Ewan Smith, Ian Collins
Jul 9, 2016·Archives of Pharmacal Research·Heesu Lee, Jae Wook Lee
Dec 17, 2016·Chemical Reviews·György DormánGlenn D Prestwich
Aug 14, 2018·Journal of Labelled Compounds & Radiopharmaceuticals·Seth JonesVolker Derdau
May 22, 2008·Molecular BioSystems·Yoshihito TanakaJennifer J Kohler
Mar 30, 2016·Chemical & Pharmaceutical Bulletin·Yuichi MasudaTakayuki Doi
May 11, 2012·Organic & Biomolecular Chemistry·Martin GolkowskiThomas Ziegler
Dec 1, 2009·Current Protocols in Chemical Biology·Andrew L Mackinnon, Jack Taunton
Apr 22, 2009·Journal of Medicinal Chemistry·Mathias Q MüllerAndrea Sinz
Nov 29, 2007·Organic Letters·Thomas DurekStephen B H Kent
Jan 24, 2009·Journal of Medicinal Chemistry·Laurence BalasSomnath Mukhopadhyay
Nov 17, 2010·ACS Chemical Biology·Brett LomenickJing Huang
Dec 14, 2016·Organic Letters·Irina ProtasovaStefan Bräse

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
Yutaka Sadakane
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
Yutaka Sadakane, Yasumaru Hatanaka
Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]
Takenori TomohiroYasumaru Hatanaka
© 2021 Meta ULC. All rights reserved