Photochemical treatment of platelet concentrates with amotosalen and long-wavelength ultraviolet light inactivates a broad spectrum of pathogenic bacteria

Transfusion
Lily LinLaurence Corash

Abstract

Bacterial contamination of platelet (PLT) concentrates can result in transfusion-transmitted sepsis. A photochemical treatment (PCT) process with amotosalen HCl and long-wavelength ultraviolet light (UVA), which cross-links nucleic acids, was developed to inactivate bacteria and other pathogens in PLT concentrates. High titers of pathogenic aerobic and anaerobic Gram-positive bacteria (10 species), aerobic Gram-negative bacteria (7 species), and spirochetes (2 species) were added to single-donor PLT concentrates containing 3.0 x 10(11) to 6.0 x 10(11) PLTs in approximately 300 mL of 35 percent plasma and 65 percent PLT additive solution (InterSol, Baxter Healthcare) or saline. After PCT with 150 micro mol per L amotosalen and 3 J per cm(2) UVA, residual bacterial levels were detected by sensitive microbiologic methods. The level of inactivation of viable bacteria was expressed as log reduction. Log reduction of Gram-positive bacteria for Staphylococcus epidermidis was > 6.6; for Staphylococcus aureus, 6.6; for Streptococcus pyogenes, > 6.8; for Listeria monocytogenes, > 6.3; for Corynebacterium minutissimum, > 6.3; for Bacillus cereus (vegetative), > 5.5; for Lactobacillus sp., > 6.4; for Bifidobacterium adolescentis, > 6.0; fo...Continue Reading

References

Jan 1, 1991·Transfusion Medicine Reviews·M Goldman, M A Blajchman
Jul 24, 1991·JAMA : the Journal of the American Medical Association·J F MorrowA K Fuller
Sep 1, 1986·The American Journal of Medicine·K C AndersonB Sullivan
Feb 11, 1999·The New England Journal of Medicine·L T GoodnoughJ P AuBuchon
Aug 9, 2001·Transfusion·P J Schmidt
Mar 16, 2002·Transfusion·Sharyn L OrtonUNKNOWN ARCNET Epidemiology Group
Jul 19, 2003·Transfusion·Theresa HarringtonAnthony A Marfin
Dec 23, 2003·Transfusion Medicine Reviews·Morris A BlajchmanFederico Baeza

❮ Previous
Next ❯

Citations

May 17, 2005·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·P MorelF Pouthier
Nov 8, 2011·Photochemistry and Photobiology·Jose-Luis SagripantiHans-Jürgen Marschall
Feb 11, 2012·Clinical and Vaccine Immunology : CVI·Kevin W BruhnNoah Craft
Jul 23, 2011·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Johannes Irsch, Lily Lin
Jul 23, 2011·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Reinhard HenschlerNina Mufti
Oct 22, 2011·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Thomas H MüllerAxel W Seltsam
Dec 24, 2004·International Journal of Hematology·Ulf Staginnus, Laurence Corash
Apr 30, 2013·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·P MorelL Bardiaux
Apr 30, 2013·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·P MorelL Bardiaux
Jul 5, 2011·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·G Andreu
Apr 9, 2011·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·S Clément
Jun 2, 2010·Clinics in Laboratory Medicine·Monique P Gelderman, Jaroslav G Vostal
Oct 2, 2009·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·John McClaskeyChristopher A Tormey
Aug 25, 2009·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Nahreen Tynngård
Sep 12, 2009·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Jaisy Mathai
May 5, 2010·Journal of Refractive Surgery·George F MangiorisTheo Seiler
Apr 19, 2008·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·P F van der Meer
Mar 21, 2008·Transfusion Medicine Reviews·Heather L ReddyRaymond P Goodrich
Feb 26, 2008·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Nahreen TynngårdMona Hansson
Dec 27, 2005·Best Practice & Research. Clinical Haematology·J P R PelletierE L Snyder
Feb 18, 2005·Journal of Internal Medicine·H G Klein
Apr 28, 2009·Transfusion·Harvey G KleinUNKNOWN NHLBI Working Group on Research Opportunities for the Pathogen Reduction/Inactivation of Blood Components
Jan 31, 2006·Transfusion·Ramon E GuevaraLaurene Mascola

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.