Photochemical treatment with endosomally localized photosensitizers enhances the number of adenoviruses in the nucleus

The Journal of Gene Medicine
B EngesaeterG M Maelandsmo

Abstract

In the present study the physical targeting technique photochemical internalization (PCI) has been used in combination with adenovirus. We have previously shown that PCI enhances transgene expression from AdhCMV-lacZ, and the aim of the present study was to further increase the understanding of photochemically mediated adenoviral transduction. Two colorectal carcinoma cell lines, WiDr and HCT116, were pre-incubated with the photosensitizer TPPS(2a) or methylene blue derivates (MBD) followed by infection with adenovirus and light exposure. Transgene expression was measured by flow cytometry. Real-time polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) were used to quantify the level of viral DNA in the nuclei. Real-time PCR was also used to measure the level of beta-galactosidase mRNA in samples infected with AdhCMV-lacZ. Exposing TPPS(2a)-treated cells to light enhanced the quantity of viral DNA in the nucleus, the mRNA level of the transgene and the transgene expression compared to non-illuminated cells. The increased transgene expression was independent of the promoter used, but dependent on the time of light exposure and the cellular localization of the photosensitizer. The enhanced transgene expre...Continue Reading

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Citations

Sep 13, 2013·Therapeutic Delivery·Sigurd Leinæs Bøe, Eivind Hovig
Jul 28, 2007·International Journal of Pharmaceutics·Stéphanie BonneauDaniel Brault
Jun 1, 2007·Photodiagnosis and Photodynamic Therapy·Asta Juzeniene, Johan Moan
Jul 6, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Pål Kristian SelboKristian Berg
Aug 10, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Arno WieheMathias O Senge
Aug 12, 2006·The Journal of Pharmacology and Experimental Therapeutics·Pål K SelboKristian Berg

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