Photochemistry on the Space Station-Antibody Resistance to Space Conditions after Exposure Outside the International Space Station

Astrobiology
Gaëlle CoussotMichel Dobrijevic

Abstract

Antibody-based analytical instruments are under development to detect signatures of life on planetary bodies. Antibodies are molecular recognition reagents able to detect their target at sub-nanomolar concentrations, with high affinity and specificity. Studying antibody binding performances under space conditions is mandatory to convince space agencies of the adequacy of this promising tool for planetary exploration. To complement previous ground-based experiments on antibody resistance to simulated irradiation, we evaluate in this paper the effects of antibody exposure to real space conditions during the EXPOSE-R2 mission outside the International Space Station. The absorbed dose of ionizing radiation recorded during the 588 days of this mission (220 mGy) corresponded to the absorbed dose expected during a mission to Mars. Moreover, samples faced, at the same time as irradiation, thermal cycles, launch constraints, and long-term storage. A model biochip was used in this study with antibodies in freeze-dried form and under two formats: free or covalently grafted to a solid surface. We found that antibody-binding performances were not significantly affected by cosmic radiation, and more than 40% of the exposed antibody, independ...Continue Reading

References

Oct 26, 2011·Analytica Chimica Acta·Thomas MoreauOdile Vandenabeele-Trambouze
Jun 12, 2012·Astrobiology·Thomas BergerGünther Reitz
Apr 9, 2013·Astrobiology·Christopher P McKayWayne H Pollard
Sep 2, 2017·Frontiers in Microbiology·Elke RabbowRainer Willnecker
Feb 8, 2018·Journal of Pharmaceutical and Biomedical Analysis·Gaëlle CoussotMichel Dobrijevic
Feb 27, 2018·Analytical Biochemistry·Gaëlle CoussotMichel Dobrijevic

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Methods Mentioned

BETA
biochips
enzyme-linked immunosorbent assays
ISS

Software Mentioned

EXPOSE
BiOMAS
R
R2

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