Photochemistry on the Space Station-Aptamer Resistance to Space Conditions: Particles Exposure from Irradiation Facilities and Real Exposure Outside the International Space Station

Astrobiology
G CoussotMichel Dobrijevic

Abstract

Some microarray-based instruments that use bioaffinity receptors such as antibodies or aptamers are under development to detect signatures of past or present life on planetary bodies. Studying the resistance of such instruments against space constraints and cosmic rays in particular is a prerequisite. We used several ground-based facilities to study the resistance of aptamers to various types of particles (protons, electrons, neutrons, and carbon ions) at different energies and fluences. We also tested the resistance of aptamers during the EXPOSE-R2 mission outside the International Space Station (ISS). The accumulated dose measured after the 588 days of this mission (220 mGy) corresponds to the accumulated dose that can be expected during a mission to Mars. We found that the recognition ability of fluorescently labeled aptamers was not significantly affected during short-term exposure experiments taking into account only one type of radiation at a time. However, we demonstrated that the same fluorescent dye was significantly affected by temperature variations (-21°C to +58°C) and storage throughout the entirety of the ISS experiment (60% of signal loss). This induced a large variability of aptamer signal in our analysis. Howev...Continue Reading

References

Jul 28, 2009·Analytical Chemistry·Josephine RutaEric Peyrin
Dec 26, 2009·Biosensors & Bioelectronics·Sandrine PerrierEric Peyrin
Mar 1, 2012·Sensors·Kyung-Mi SongChangill Ban
Jun 12, 2012·Astrobiology·Thomas BergerGünther Reitz
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Mar 4, 2014·Critical Reviews in Food Science and Nutrition·Yiyang DongDaning Wang
Dec 3, 2015·Expert Opinion on Drug Discovery·Guido Tomás RozenblumMartín Radrizzani
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Feb 27, 2018·Analytical Biochemistry·Gaëlle CoussotMichel Dobrijevic

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Methods Mentioned

BETA
biochip
biosensors
ISS

Software Mentioned

Geant4
TableCurve
EXPOSE
R2

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