Photochemotherapy inhibits angiogenesis and induces apoptosis of endothelial cells in vitro

Photodermatology, Photoimmunology & Photomedicine
Hui DengKang-huang Liao

Abstract

Photochemotherapy has long been used in the treatment of psoriasis; however, its mechanism has not been completely elucidated. Psoriasis is now regarded as an angiogenesis-related disease. Recent studies indicated that the inhibition of angiogenesis by photochemotherapy could be an underlying mechanism. It was found that photochemotherapy can downregulate the expression of angiogenic factors in keratinocytes. However, the direct effect of photochemotherapy on endothelial cells has not been studied. In this study, we determined the effect of photochemotherapy on the proliferation of human microvascular endothelial cells through MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and cell cycle analysis. The migration assay and in vitro tube formation assay were used to investigate the migration properties and tube formation ability of human microvascular endothelial cells after psoralen plus UVA (PUVA) treatment. The apoptosis of endothelial cells elicited by photochemotherapy was also analyzed with fluorescence-activated cell sorting analysis (FACS). UVA (0.8-5.0 J/cm(2)) irradiation with the presence of 8-methoxypsoralen (8-MOP) (300 ng/ml) resulted in a dose-dependent reduction in the cell viabilities of ...Continue Reading

References

Jan 1, 1992·The British Journal of Dermatology·B S Baker, L Fry
Feb 1, 1989·The American Journal of Dermatopathology·V N Mordovtsev, V I Albanova
Jan 1, 1986·Clinical Pharmacokinetics·F A de Wolff, T V Thomas
Dec 1, 1985·The Journal of Investigative Dermatology·G D WeinsteinP A Ross
Jan 1, 1983·Journal of Ultrastructure Research·K G BenschM A Karasek
Oct 1, 1981·The Journal of Investigative Dermatology·H Okamoto, T Horio
Apr 1, 1981·The British Journal of Dermatology·W L MorisonJ I Krugler
Jan 1, 1982·The Journal of Investigative Dermatology·I M Braverman, J Sibley
Feb 1, 1994·The British Journal of Dermatology
May 1, 1996·Photochemistry and Photobiology·F P Gasparro
Sep 18, 1998·The Journal of Investigative Dermatology·M LüftlK Degitz
Feb 17, 1999·Photodermatology, Photoimmunology & Photomedicine·T R CovenJ G Krueger
Mar 31, 1999·Journal of Dermatological Science·D BetheaF P Gasparro
Jun 3, 1999·The British Journal of Dermatology·M S DuthieM Norval
Mar 31, 2000·Nature Medicine·P Carmeliet
Apr 15, 2000·Journal of Dermatological Science·T Horio
Sep 23, 2000·Nature·P Carmeliet, R K Jain
Mar 27, 2001·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·G CaiD A Beacham
May 12, 2001·The Journal of Investigative Dermatology·K KurodaM Lebwohl
Jun 26, 2001·Clinical and Experimental Dermatology·H Hönigsmann
Nov 13, 2001·American Journal of Clinical Dermatology·F P Gasparro
Sep 5, 2002·The British Journal of Dermatology·M BhushanC E M Griffiths
Nov 2, 2002·The British Journal of Dermatology·P Velasco, B Lange-Asschenfeldt
Dec 28, 2002·The Journal of Biological Chemistry·Yu-Ying HeColin F Chignell

❮ Previous
Next ❯

Citations

Mar 19, 2013·Cell Biochemistry and Biophysics·Olfat G ShakerAli Ashmaui
Nov 18, 2011·Expert Opinion on Investigational Drugs·A A CrawshawH S Young
Feb 13, 2016·Expert Opinion on Investigational Drugs·N Malecic, H S Young
Oct 2, 2007·Dermatologic Clinics·Hagit Matz
May 15, 2008·Photodermatology, Photoimmunology & Photomedicine·Ayse AkmanErtan Yilmaz
Jan 10, 2012·Journal of the European Academy of Dermatology and Venereology : JEADV·N H El-EishiL Rashed
Jul 13, 2020·Experimental Dermatology·Andrea Luengas-MartinezHelen S Young

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis