Photochromic biliproteins from the cyanobacterium Anabaena sp. PCC 7120: lyase activities, chromophore exchange, and photochromism in phytochrome AphA

Biochemistry
Kai-Hong ZhaoH Scheer

Abstract

Photochromic biliproteins can be switched by light between two states, initiated by Z/E photoisomerization of the linear tetrapyrrole chromophore. The cyanobacterium Anabaena sp. PCC 7120 contains three genes coding for such biliproteins, two coding for phytochromes (aphA/B) and one for the alpha subunit of phycoerythrocyanin (pecA). (a) aphA was overexpressed in Escherichia coli with N-terminal His and S tags, and the protein was reconstituted by an optimized protocol with phycocyanobilin (PCB), to yield the photochromic chromoprotein, PCB-AphA, carrying the PCB chromophore. (b) AphA chromophorylation is autocatalytic such as in other phytochromes. (c) AphA chromophorylation is also possible by chromophore transfer from the PCB-carrying biliprotein, phycocyanin (CPC). The autocatalytic transfer is very slow, and it is enhanced more than 100-fold by catalysis of PCB:CpcA lyase and alpha-CPC as donor. (d) Through deletion mutations of aphA, a short sequence IQPHGV [amino acids (aa) 26-31] was found essential for the lyase activity of AphA, indicating an interaction of the N terminus with the chromophore-binding domain around cysteine 259. (e) A motif of at least 23 aa, starting with this sequence and located approximately 250 aa...Continue Reading

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Citations

Mar 28, 2009·Journal of the American Chemical Society·Jun-Ming TuKai-Hong Zhao
Oct 11, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·A Losi, W Gärtner
Mar 8, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Carmen MandalariWolfgang Gärtner
Oct 30, 2009·The Journal of Biological Chemistry·Michaela KupkaKai-Hong Zhao
Sep 30, 2010·The Journal of Biological Chemistry·Jessica WiethausNicole Frankenberg-Dinkel
Aug 24, 2007·Plant & Cell Physiology·Takami IshizukaMasahiko Ikeuchi
Jan 3, 2006·The Plant Cell·Nathan C Rockwell, J Clark Lagarias
May 4, 2006·Annual Review of Plant Biology·Nathan C RockwellJ Clark Lagarias
Aug 30, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kai-Hong ZhaoHugo Scheer
Jun 24, 2008·Journal of Molecular Biology·Rei NarikawaMasahiko Ikeuchi
Mar 24, 2016·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Che YuanKai-Hong Zhao
Dec 29, 2004·Biochimica Et Biophysica Acta·Kai-Hong ZhaoHugo Scheer
Jun 26, 2008·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Jian ZhangJoseph R Lakowicz
Nov 29, 2017·Proceedings of the National Academy of Sciences of the United States of America·Cheng ZhaoKai-Hong Zhao
Feb 21, 2008·Molecular Microbiology·H Scheer, K-H Zhao
Jun 28, 2007·The Journal of Biological Chemistry·Stephan BöhmKai-Hong Zhao
Jan 22, 2011·Critical Reviews in Biochemistry and Molecular Biology·Michele E Auldridge, Katrina T Forest
May 14, 2017·Photochemistry and Photobiology·Lu LuKai-Hong Zhao

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