PMID: 15377271Sep 21, 2004Paper

Photoconversion of the chromophore of a fluorescent protein from Dendronephthya sp

Biochemistry. Biokhimii︠a︡
A A PakhomovVladimir I Martynov

Abstract

A green fluorescent protein from the coral Dendronephthya sp. (Dend FP) is characterized by an irreversible light-dependent conversion to a red-emitting form. The molecular basis of this phenomenon was studied in the present work. Upon UV-irradiation at 366 nm, the absorption maximum of the protein shifted from 494 nm (the green form) to 557 nm (the red form). Concurrently, in the fluorescence spectra the emission maximum shifted from 508 to 575 nm. The green form of native Dend FP was shown to be a dimer, and the oligomerization state of the protein did not change during its conversion to the red form. By contrast, UV-irradiation caused significant intramolecular changes. Unlike the green form, which migrates in SDS-polyacrylamide gels as a single band corresponding to a full-length 28-kD protein, the red form of Dend FP migrated as two fragments of 18- and 10-kD. To determine the chemical basis of these events, the denatured red form of Dend FP was subjected to proteolysis with trypsin. From the resulting hydrolyzate, a chromophore-containing peptide was isolated by HPLC. The structure of the chromophore from the Dend FP red form was established by methods of ESI, tandem mass spectrometry (ESI/MS/MS), and NMR-spectroscopy. Th...Continue Reading

Citations

Jun 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Karin NienhausHerbert Nar
Jan 30, 2008·Current Protocols in Cell Biology·Scott G OlenychMichael W Davidson
Apr 15, 2009·Biochemistry. Biokhimii︠a︡·A A Pakhomov, V I Martynov
Sep 23, 2009·Chemical Society Reviews·Richard N Day, Michael W Davidson
Sep 19, 2015·PloS One·Minrong AiGreg Suh
Apr 30, 2016·Bioorganicheskaia khimiia·A A PakhomovV I Martynov
Jul 30, 2010·Biochemistry·Rebekka M WachterHanseong Kim
Aug 30, 2008·Chemistry & Biology·Alexey A Pakhomov, Vladimir I Martynov

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