Photocrosslinked layered gelatin-chitosan hydrogel with graded compositions for osteochondral defect repair

Journal of Materials Science. Materials in Medicine
Fengxuan HanXiaoyan Yuan

Abstract

A layered gelatin-chitosan hydrogel with graded composition was prepared via photocrosslinking to simulate the polysaccharide/collagen composition of the natural tissue and mimic the multi-layered gradient structure of the cartilage-bone interface tissue. Firstly, gelatin and carboxymethyl chitosan were reacted with glycidyl methacrylate (GMA) to obtain methacrylated gelatin (Gtn-GMA) and carboxymethyl chitosan (CS-GMA). Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties. Results suggested that the pore sizes of the superficial, transitional and deep layers of the layered hydrogel were 115 ± 30, 94 ± 34, 51 ± 12 μm, respectively and their porosities were all higher than 80 %. The compressive strengths of them were 165 ± 54, 565 ± 50 and 993 ± 108 kPa, respectively and the strain of the gradient hydrogel decreased along the thickness direction, similar to the natural tissue. The in vitro cytotoxicity results showed that the hydrogel had good cyt...Continue Reading

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Citations

Jan 20, 2018·Regenerative Medicine·Ji XiongfaXiao Jun
Apr 25, 2016·Journal of Materials Science. Materials in Medicine·Yang YangYubo Fan
Apr 2, 2021·Biomacromolecules·Erwan Nicol
Jul 9, 2018·ACS Biomaterials Science & Engineering·Zihao Xu, Kaitlin M Bratlie
Sep 14, 2015·ACS Biomaterials Science & Engineering·C A VilelaR L Reis
Feb 11, 2019·ACS Biomaterials Science & Engineering·Gülseren IrmakMenemşe Gümüşderelioǧlu

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