Photodithazine photodynamic effect on viability of 9L/lacZ gliosarcoma cell line

Lasers in Medical Science
Letícia Correa FontanaJuliana Ferreira-Strixino

Abstract

Even with the advances of conventional treatment techniques, the nervous system cancer prognosis is still not favorable to the patient which makes alternative therapies needed to be studied. Photodynamic therapy (PDT) is presented as a promising therapy, which employs a photosensitive (PS) agent, light wavelength suitable for the PS agent, and molecular oxygen, producing reactive oxygen species in order to induce cell death. The aim of this study is to observe the PDT action in gliosarcoma cell using a chlorin (Photodithazine, PDZ). The experiments were done with 9L/lacZ lineage cells, grown in a DMEM medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution and put in a culture chamber at 37 °C with an atmosphere of 5% CO2. The PS agent used was the PDZ to an LED light source device (Biopdi/IRRAD-LED 660) in the 660-nm region. The location of the PS agent was analyzed by fluorescence microscopy, and cell viability was analyzed by MTT assay (mitochondrial activity), exclusion by trypan blue (cell viability), and morphological examination through an optical microscope (Leica MD 2500). In the analysis of the experiments with PDZ, there was 100% cell death at different concentrations and clear morphol...Continue Reading

References

Dec 6, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Junkoh YamamotoHiroki Namba
Sep 15, 2009·Journal of Neurosurgery·Abraham TsitlakidisPanagiotis Selviaridis
May 8, 2010·Neurologia Medico-chirurgica·Daisuke MaedaKatsuji Shima
Jan 25, 2011·Photomedicine and Laser Surgery·Luis Fernando TirapelliCarlos Gilberto Carlotti Junior
May 28, 2011·CA: a Cancer Journal for Clinicians·Patrizia AgostinisJakub Golab
Apr 3, 2012·Lasers in Medical Science·Carla R FontanaNikolaos S Soukos
Jun 14, 2013·Biological Research·Qunwen PanBin Liang
Sep 26, 2013·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Jonathan P MannasJ Lee Villano
Jun 4, 2014·Journal of Photochemistry and Photobiology. B, Biology·H H BuzzáC Kurachi
Nov 7, 2014·The Journal of Toxicological Sciences·Yuichi MikiYasuyuki Fujiwara

❮ Previous
Next ❯

Citations

Jan 25, 2019·Experimental Biology and Medicine·Carlos Dailton Guedes de Oliveira MoraesCristina Pacheco Soares
Feb 13, 2020·Photodiagnosis and Photodynamic Therapy·Rainara Moreno Sanches de AlmeidaJuliana Ferreira-Strixino
Feb 28, 2021·Photodiagnosis and Photodynamic Therapy·Gabrielle Dos Santos VitorioJuliana Ferreira-Strixino

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

© 2022 Meta ULC. All rights reserved