Photodynamic and Antibiotic Therapy in Combination to Fight Biofilms and Resistant Surface Bacterial Infections

International Journal of Molecular Sciences
Federica BarraMaria Rosaria Catania

Abstract

Although photodynamic therapy (PDT), a therapeutic approach that involves a photosensitizer, light and O₂, has been principally considered for the treatment of specific types of cancers, other applications exist, including the treatment of infections. Unfortunately, PDT does not always guarantee full success since it exerts lethal effects only in cells that have taken up a sufficient amount of photosensitizer and have been exposed to adequate light doses, conditions that are not always achieved. Based on our previous experience on the combination PDT/chemotherapy, we have explored the possibility of fighting bacteria that commonly crowd infected surfaces by combining PDT with an antibiotic, which normally does not harm the strain at low concentrations. To this purpose, we employed 5-aminolevulinic acid (5-ALA), a pro-drug that, once absorbed by proliferating bacteria, is converted into the natural photosensitizer Protoporphyrin IX (PpIX), followed by Gentamicin. Photoactivation generates reactive oxygen species (ROS) which damage or kill the cell, while Gentamicin, even at low doses, ends the work. Our experiments, in combination, have been highly successful against biofilms produced by several Gram positive bacteria (i.e., Sta...Continue Reading

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Citations

May 21, 2016·Frontiers in Microbiology·Sylvie MiquelChristiane Forestier
May 5, 2016·Photodiagnosis and Photodynamic Therapy·Hang ShiHongying Sun
Jan 16, 2018·Lasers in Surgery and Medicine·Natanel IluzZvi Malik
Mar 2, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Vanesa Pérez-LagunaMichael R Hamblin
Jul 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bamidele M Amos-TautuaOluwatobi S Oluwafemi
Mar 9, 2019·International Journal of Molecular Sciences·Chun-Chen YangChun-Han Hou
Dec 6, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yana IlizirovMarina Nisnevitch
Jun 6, 2018·Frontiers in Microbiology·Agata Wozniak, Mariusz Grinholc
Feb 29, 2020·Photodiagnosis and Photodynamic Therapy·Christiane Costa MagachoJuliana Ferreira-Strixino
May 11, 2020·Biochemical Pharmacology·Gabriela Cervini BohmAdriana Casas
Apr 10, 2021·Photodiagnosis and Photodynamic Therapy·Camila PérezChristian Erick Palavecino
Jul 9, 2021·Frontiers in Microbiology·María Paulina RomeroVanderlei Salvador Bagnato
Aug 8, 2021·International Journal of Molecular Sciences·Ana C AfonsoManuel Simões
Aug 19, 2020·ACS Applied Materials & Interfaces·Lin MeiJunxia Zhang

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Methods Mentioned

BETA
confocal microscopy
Laser Scanner Microscopy
Assay
ELISA
Scanning Microscopy

Software Mentioned

IMARIS
BITPLANE
R Development Core Team

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