PMID: 8992507Jun 1, 1996Paper

Photodynamic imaging of a rat pancreatic cancer with pheophorbide a

Photochemistry and Photobiology
P KellerM Aprahamian

Abstract

Laser-induced fluorescence of pheophorbide a (Ph-a) was used for in vitro photodynamic imaging (PDI) of a rat pancreatic acinar tumor. A 400 nm excitation induced a 470 nm autofluorescence and a 678 nm dye fluorescence in tumors and their surrounding pancreas 24 h after a 9 mg kg-1 body weight Ph-a intravenous administration. With lower intensities in these blood-rich tumors than in pancreas, Ph-a fluorescence signals are unable to provide tumor images. A dimensionless function (the ratio of Ph-a fluorescence by autofluorescence, called Rt for the tumor and Rp for the pancreas) was used for fluorescence contrast calculation (C = Rt/Rp) between six tumors and their paired pancreas. Among five available laser excitation wave-lengths, only the 355 nm excitation gave a distinctive contrast (C = 1.5). The PDI of six intrapancreatic tumors and their intraperitoneal metastasis and of two control normal pancreas was thus performed ex vivo using a 355 nm excitation source delivered by a tripled Nd:YAG laser and a charged-coupled device camera. Fluorescence images were recorded at 680 nm (dye), 640 nm (background) and 470 nm (autofluorescence) through three corresponding 10 nm width bandpass filters. Computed division for each pixel of P...Continue Reading

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Citations

Jan 21, 2004·Journal of Photochemistry and Photobiology. B, Biology·Emma S Nyman, Paavo H Hynninen
Apr 29, 2008·Gastrointestinal Endoscopy·Herbert C Wolfsen
Jan 6, 2017·Organic & Biomolecular Chemistry·Anjul KhadriaHarry L Anderson
Oct 31, 2001·Photochemistry and Photobiology·A ZimmermannH Kostron
Jun 1, 1997·Photochemistry and Photobiology·V TassettiM Aprahamian
Jul 10, 2019·International Journal of Molecular Sciences·Manuel Gallardo-VillagránBruno Therrien

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