Photodynamic inactivation of recombinant bioluminescent Escherichia coli by cationic porphyrins under artificial and solar irradiation.

Journal of Industrial Microbiology & Biotechnology
Eliana AlvesAdelaide Almeida

Abstract

A faster and simpler method to monitor the photoinactivation process of Escherichia coli involving the use of recombinant bioluminescent bacteria is described here. Escherichia coli cells were transformed with luxCDABE genes from the marine bioluminescent bacterium Vibrio fischeri and the recombinant bioluminescent indicator strain was used to assess, in real time, the effect of three cationic meso-substituted porphyrin derivatives on their metabolic activity, under artificial (40 W m(-2)) and solar irradiation (approximately 620 W m(-2)). The photoinactivation of bioluminescent E. coli is effective (>4 log bioluminescence decrease) with the three porphyrins used, the tricationic porphyrin Tri-Py+-Me-PF being the most efficient compound. The photoinactivation process is efficient both with solar and artificial light, for the three porphyrins tested. The results show that bioluminescence analysis is an efficient and sensitive approach being, in addition, more affordable, faster, cheaper and much less laborious than conventional methods. This approach can be used as a screening method for bacterial photoinactivation studies in vitro and also for the monitoring of the efficiency of novel photosensitizer molecules. As far as we kno...Continue Reading

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Citations

Nov 1, 2011·Applied Microbiology and Biotechnology·Eliana AlvesAdelaide Almeida
Feb 16, 2012·Photochemistry and Photobiology·Joana B PereiraJoão P C Tomé
Jan 29, 2014·Future Medicinal Chemistry·Eliana AlvesAdelaide Almeida
Apr 8, 2014·Journal of Photochemistry and Photobiology. B, Biology·L MamoneA Casas
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Mar 26, 2016·Frontiers in Microbiology·Maria BartolomeuAdelaide Almeida
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Mar 7, 2017·Future Medicinal Chemistry·Lúcia MarcielAdelaide Almeida
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