Photodynamic therapy associating Photogem and blue LED on L929 and MDPC-23 cell culture

Cell Biology International
Ana Paula Dias RibeiroCarlos Alberto de Souza Costa

Abstract

To evaluate the cytotoxicity of PDT (photodynamic therapy) with Photogem associated to blue LED (light-emitting diode) on L929 and MDPC-23 cell cultures, 30000 cells/cm2 were seeded in 24-well plates for 48 h, incubated with Photogem (10, 25 or 50 mg/l) and irradiated with an LED source (460+/-3 nm; 22 mW/cm2) at two energy densities (25.5 or 37.5 J/cm2). Cell metabolism was evaluated by the MTT (methyltetrazolium) assay (Dunnet's post hoc tests) and cell morphology by SEM (scanning electron microscopy). Flow cytometry analysed the type of PDT-induced cell death as well and estimated intracellular production of ROS (reactive oxygen species). There was a statistically significant decrease of mitochondrial activity (90% to 97%) for all Photogem concentrations associated to blue LED, regardless of irradiation time. It was also demonstrated that the mitochondrial activity was not recovered after 12 or 24 h, characterizing irreversible cell damage. PDT-treated cells presented an altered morphology with ill-defined limits. In both cell lines, there was a predominance of necrotic cell death and the presence of Photogem or irradiation increased the intracellular levels of ROS. PDT caused severe toxic effects in normal cell culture, cha...Continue Reading

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Citations

Apr 27, 2011·Photochemistry and Photobiology·Lívia N DovigoCristina Kurachi
Feb 24, 2012·Lasers in Medical Science·Ana Paula Dias RibeiroCarlos Alberto de Souza Costa
Apr 15, 2014·Photodiagnosis and Photodynamic Therapy·Ivana Márcia Alves DinizMaria Esperanza Cortés
Apr 26, 2016·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Claudia Viviane Guimarães PellissariJanaina Habib Jorge

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