PMID: 9434126Jan 20, 1998Paper

Photodynamic therapy inhibits cell adhesion without altering integrin expression

Biochimica Et Biophysica Acta
P MargaronJ G Levy

Abstract

Adhesion is a primordial cell function that, among others, regulates inflammation, metastasis, and tissue repair. To understand how these events could be affected by photodynamic therapy (PDT), we studied the effects of PDT on human foreskin fibroblast (HFF) adhesion to bovine collagen type I, human vitronectin or fibronectin. PDT, using benzoporphyrin derivative monoacid ring A (verteporfin) as the photosensitizer, inhibited cell adhesion in a drug dose-dependent manner, with no significant difference among matrices. The drug dose that killed 90% of cells within 20 h post-treatment inhibited HFF adhesion by 55%-68%. However, 45 min following PDT, a time period corresponding to that of the adhesion assay, HFF membrane integrity remained unaltered. In addition, cell surface expression of integrins was not modified for at least 2h following PDT. Western blots of cell lysates, using the anti-phosphotyrosine 4G10 monoclonal antibody, revealed that PDT prevented the adhesion-induced phosphorylation of 110-130 kDa proteins. Immunoblots of cell lysates immunoprecipitated with antibodies to focal adhesion kinase suggested that its phosphorylation was suppressed by PDT. These results demonstrate that PDT inhibits cell adhesion and affec...Continue Reading

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Citations

Jan 20, 2004·Biochimica Et Biophysica Acta·Anatoly UzdenskyJohan Moan
Mar 29, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Lawrence G SullivanChristine A Towle
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Oct 11, 2012·Biochimica Et Biophysica Acta·Laura Milla SanabriaViviana Alicia Rivarola
Jun 1, 2007·Photodiagnosis and Photodynamic Therapy·Asta Juzeniene, Johan Moan
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Feb 18, 2005·Biochimica Et Biophysica Acta·Anatoly UzdenskyJohan Moan
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Jan 25, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Małgorzata FirczukJakub Gołąb
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Jul 21, 2016·Journal of Cellular Biochemistry·Gustavo CalvoGabriela Di Venosa

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