Photodynamic therapy mediated by chlorin-type photosensitizers against Streptococcus mutans biofilms

Photodiagnosis and Photodynamic Therapy
Maíra Terra GarciaJuliana Campos Junqueira

Abstract

Photodynamic therapy (PDT) can be used for the control of oral pathogens and different photosensitizers (PS) have been investigated. This study evaluated the efficacy of PDT against Streptococcus mutans biofilms using two second-generation PS derived from chlorin: Photoditazine® (PDZ) and Fotoenticine® (FTC). These PS were compared to methylene blue (MB), a dye with proven antimicrobial activity against S. mutans. Suspensions of S. mutans were cultured in contact with bovine tooth disks for biofilm formation. After 48 h, the biofilms were treated with PDZ (0.6 mg/mL), FTC (0.6 mg/mL) or MB (1 mg/mL) and submitted to laser irradiation (660 nm, 50 mW/cm2). The biofilms were quantified by the determination of CFU/mL count and analyzed by scanning electron microscopy (SEM). All PS used for PDT reduced the number of S. mutans, with a statistically significant difference compared to the untreated groups. PDT achieved microbial reductions of 4 log with MB and 6 log with PDZ, while the use of FTC resulted in the complete elimination of S. mutans biofilms. SEM analysis confirmed the CFU/mL results, showing that all PS, particularly FTC, were able to detach the biofilms and to eliminate the bacteria. In conclusion, PDT mediated by chlori...Continue Reading

Citations

Jul 26, 2019·International Journal of Molecular Sciences·Analú Barros de OliveiraCarla Raquel Fontana
Feb 29, 2020·Photodiagnosis and Photodynamic Therapy·Christiane Costa MagachoJuliana Ferreira-Strixino
Nov 7, 2020·Photodiagnosis and Photodynamic Therapy·Anjali WarrierThokur Sreepathy Murali
Sep 23, 2020·Photodiagnosis and Photodynamic Therapy·Cheyenne Marçal de SouzaJuliana Campos Junqueira
Apr 23, 2021·Photodiagnosis and Photodynamic Therapy·Maíra Terra-GarciaJuliana Campos Junqueira
Jul 3, 2021·International Journal of Molecular Sciences·Nikita SuvorovMikhail Grin

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