Photodynamic therapy with two different photosensitizers as a supplement to instrumentation/irrigation procedures in promoting intracanal reduction of Enterococcus faecalis

Journal of Endodontics
Letícia C SouzaJosé F Siqueira

Abstract

This in vitro study aimed to investigate the antibacterial effects of photodynamic therapy (PDT) with methylene blue (MB) or toluidine blue (TB) (both at 15 microg/mL) as a supplement to instrumentation/irrigation of root canals experimentally contaminated with Enterococcus faecalis. Seventy extracted teeth had their root canals contaminated with an endodontic strain of E. faecalis for 7 days, instrumented with nickel-titanium instruments and irrigated either with 2.5% NaOCl or with 0.85% NaCl, and then randomly distributed into four experimental groups: MB/NaOCl (PDT with MB and NaOCl as the irrigant), TB/NaOCl (PDT with TB and NaOCl as the irrigant), MB/NaCl (PDT with MB and NaCl as the irrigant), and TB/NaCl (PDT with TB and NaCl as the irrigant). For PDT, the photosensitizer remained in the canal for 2 minutes before exposed to red light emitted from a diode laser for 4 minutes. Samples were taken before and after instrumentation/irrigation and following the specific PDT procedure for each group, plated onto Mitis-salivarius agar and the colony forming units counted. Regardless of the irrigant used (NaOCl or NaCl), instrumentation significantly reduced bacterial counts in comparison to the baseline (p < 0.001). NaOCl as the...Continue Reading

Citations

Dec 23, 2011·International Journal of Dentistry·Sushma Jaju, Prashant P Jaju
Jul 22, 2014·Photomedicine and Laser Surgery·Rômulo Aguiar FigueiredoDenise Pontes Raldi
Oct 22, 2013·Journal of Endodontics·Evren OkTuba Gok
Jul 6, 2011·Dental Clinics of North America·Rory E Mortman
Feb 16, 2016·Laser Therapy·Omid Heidar MuhammadEtienne Medioni
Apr 12, 2016·Journal of Photochemistry and Photobiology. B, Biology·Arash AziziArash Rahimi
Jan 20, 2016·International Journal of Dentistry·P DiogoJ M Santos
Dec 17, 2014·Journal of Oral Science·Ricardo A S ArneiroLeonardo S Antunes
Nov 29, 2011·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·José F Siqueira, Isabela N Rôças
Nov 28, 2012·Journal of Medical Microbiology·A Al-AhmadM J Altenburger
May 27, 2015·Photodiagnosis and Photodynamic Therapy·Bruna Paloma de OliveiraMonica Felts de La Roca Soares
Nov 19, 2014·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·Shipra SinghSashi Prabha Tyagi
Dec 17, 2014·Lasers in Medical Science·Matheus Franco da FrotaFabio Luis Camargo Villela Berbert
Jan 18, 2011·Journal of Endodontics·Raymond NgNikolaos S Soukos
Mar 25, 2014·Acta Odontologica Scandinavica·Ulrich SchiffnerSigrun Eick
Mar 4, 2014·Photodiagnosis and Photodynamic Therapy·Omid H MuhammadEtienne Medioni
Apr 27, 2016·Journal of Photochemistry and Photobiology. B, Biology·Larissa Menezes CostaMaria Amália Gonzaga Ribeiro
Dec 3, 2014·Photodiagnosis and Photodynamic Therapy·Prasanna NeelakantanAnil Kishen
Nov 6, 2014·Case Reports in Dentistry·Patricia da Silva BarbosaGiselle Rodrigues de Sant' Anna
Feb 27, 2015·Photomedicine and Laser Surgery·Alessandra Cesar TrindadeJoão Batista Blessmann Weber
Jun 23, 2016·Journal of Lasers in Medical Sciences·Nasim ChiniforushAbbas Bahador
Aug 27, 2016·Photomedicine and Laser Surgery·Sérgio Luiz PinheiroCarlos Eduardo da Silveira Bueno
Sep 3, 2016·Photodiagnosis and Photodynamic Therapy·Mayara Santos de CastroJoão Adolfo Costa Hanemann
Aug 8, 2014·Photodiagnosis and Photodynamic Therapy·Guilherme Cavagnoli GhinzelliJosé Antônio Poli de Figueiredo
Jul 23, 2015·Brazilian Dental Journal·Fernanda Ullmann LópezFabiana Vieira Vier-Pelisser
Mar 11, 2018·Scientific Reports·Laura Marise de FreitasCarla Raquel Fontana

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