PMID: 9523536Apr 2, 1998Paper

Photodynamic treatment with benzoporphyrin derivative monoacid ring A produces protein tyrosine phosphorylation events and DNA fragmentation in murine P815 cells

Photochemistry and Photobiology
D J GranvilleD W Hunt

Abstract

Treatment with benozopophyrin derivative monoacid ring A (BPD-MA, verteporfin) and broad-spectrum fluorescent light rapidly produced apoptosis in murine P815 mastocytoma cells. Fragmentation of DNA, a fundamental characteristic of cells undergoing apoptosis, was evident within 3 h following the photodynamic treatment. Western immunoblot analysis using the specific antiphosphotyrosine monoclonal antibody 4G10 indicated that molecular species of > 200 kDa were phosphorylated on tyrosine residues during or immediately following the irradiation of cells loaded with BPD-MA. Increased tyrosine phosphorylation of a 15 kDa protein was evident by 15 min postirradiation. In the absence of light, BPD-MA did not affect the status of cellular protein tyrosine phosphorylation or cause DNA fragmentation. The protein kinase inhibitor staurosporine prevented tyrosine phosphorylation of the > 200 kDa species but did not affect tyrosine phosphorylation of the 15 kDa protein or the level of DNA fragmentation produced by the photodynamic treatment. The protein tyrosine phosphorylation events observed for P815 cells treated with cytotoxic levels of BPD-MA and light may not be directly related to the induction of the apoptotic cell death pathway.

Citations

Sep 19, 2009·Immunologic Research·Sandra O Gollnick, Craig M Brackett
Jun 24, 1998·Journal of the National Cancer Institute·T J DoughertyQ Peng
Jun 25, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Edith KabinguSandra O Gollnick
Feb 28, 2013·Photochemistry and Photobiology·Imran RizviTayyaba Hasan
Sep 3, 2004·Journal of Photochemistry and Photobiology. B, Biology·Won-Young LeeChang-Whan Cho
Sep 15, 2004·Biochimica Et Biophysica Acta·Ramiro D AlmeidaCarlos B Duarte
Mar 29, 2006·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Stanley S Stylli, Andrew H Kaye
Apr 25, 2006·Free Radical Biology & Medicine·Galina G KramarenkoFreya Q Schafer

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis