Photoelectrochemical biosensing of disease marker on p-type Cu-doped Zn0.3 Cd0.7 S based on RCA and exonuclease III amplification

Biosensors & Bioelectronics
Kangyao ZhangDianping Tang

Abstract

In this work, a new "signal-on" split-type photoelectrochemical (PEC) sensing platform for prostate-specific antigen (PSA) detection was successfully constructed using p-type Cu-doped Zn0.3Cd0.7S as the photosensitive semiconductor material and target-triggered rolling circle amplification (RCA) for signal amplification. The signal derived from Cu-doped Zn0.3Cd0.7S was amplified by hemin/G-quadruplex. Upon target PSA introduction, the aptamer-primer probe (apt-pri) was captured by capture antibody-conjugated magnetic bead (MB-mAb) to form the sandwiched MB-mAb/PSA/apt-pri. The complex could initiate the RCA reaction to produce a long single-stranded DNA that provided binding sites for G-rich DNA and to form long single-stranded DNA/G-quadruplex/hemin. Upon the addition of exonuclease III (Exo III), the hemin/G-quadruplex immobilized on the RCA long product could be released by the digestion of Exo III. The hemin/G-quadruplex complexes in this study were used as efficient electron acceptors to neutralize the photoelectrons generated from the semiconductor and hindered the recombination of charges, thus enhancing the photocurrent. Under the optimum conditions, the developed sensing system displayed a good analytical performance...Continue Reading

Citations

Aug 9, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Liwei YangDanny K Y Wong
Mar 1, 2020·Nucleic Acids Research·Mégane DebiaisSabine Müller
Mar 16, 2021·Trends in Biotechnology·Shuzhen YueSai Bi
Sep 9, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Keshani G Gunasinghe Pattiya ArachchillageJuan M Artes Vivancos

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