Photoelectrochemical sensitive detection of insulin based on CdS/polydopamine co-sensitized WO3 nanorod and signal amplification of carbon nanotubes@polydopamine

Biosensors & Bioelectronics
Rongyu WangQin Wei

Abstract

An ultrasensitive photoelectrochemical sandwich immunosensor was designed for detection of insulin based on WO3/CdS/polydopamine (WO3/CdS/PDA) co-sensitized and PDA@carbon nanotubes (PDA@CNT) conjugates for signal amplification. The CdS nanoparticles were first deposited on the WO3 nanorods via sequential chemical bath deposition to form the WO3/CdS structure to enhance photocurrent. Then equipped with PDA to form the WO3/CdS/PDA photosensitive structure. The PDA was used not only to reduce the toxicity of CdS but also adsorb insulin primary antibodies (Ab1). Meanwhile, insulin secondary antibodies (Ab2) were decorated by PDA@CNT conjugates for signal amplification and further enhance photocurrent. Different photocurrent intensities were obtained by the photoelectrochemical workstation at applied bias of 0V due to the different amount of the PDA@CNT conjugates introduced by the different concentrations of insulin. A good linear relationship was obtained between the increased photocurrent and insulin concentrations range from 0.01ngmL-1 to 50ngmL-1. And a detection limit of 2.8pgmL-1 was obtained. The proposed sensor was applied to the determination of the insulin in human serum sample, and satisfactory results were obtained. Th...Continue Reading

Citations

Apr 25, 2018·Chemistry : a European Journal·Yang ZangHuan Pang
Aug 7, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sebastian Cajigas, Jahir Orozco
Jan 3, 2021·Colloids and Surfaces. B, Biointerfaces·Hanmei LiQiang Peng
Jun 10, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Hongmin MaQin Wei
Dec 7, 2021·Frontiers in Bioengineering and Biotechnology·Guangli HeYibiao Liu

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