Nov 19, 2019

Photoexcited Toluidine Blue Inhibits Tau Aggregation in Alzheimer's Disease

ACS Omega
Tushar DubeySubashchandrabose Chinnathambi

Abstract

The aggregates of microtubule-associated protein Tau are considered as a major hallmark of Alzheimer's disease. Tau aggregates accumulate intracellularly leading to neuronal toxicity. Numerous approaches have been targeted against Tau protein aggregation, which include application of synthetic and natural compounds. Toluidine blue is a basic dye of phenothiazine family, which on irradiation with a 630 nm light gets converted into a photoexcited form, leading to generation of singlet oxygen species. Methylene blue is the parent compound of toluidine blue, which has been reported to be potent against tauopathy. In the present work, we studied the potency of toluidine blue and photoexcited toluidine blue against Tau aggregation. Biochemical and biophysical analyses using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, ThS fluorescence, circular dichroism spectroscopy, and electron microscopy suggested that toluidine blue inhibited the aggregation of Tau in vitro. The photoexcited toluidine blue potentially dissolved the matured Tau fibrils, which indicated the disaggregation property of toluidine blue. The cell biology studies including the cytotoxicity assay and reactive oxygen species (ROS) production assay suggested ...Continue Reading

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Mentioned in this Paper

Study
Tauopathies
Cytotoxicity Assay
Polyacrylamide Gels
Natural Products
Memory Impairment
Neurons
Fetal Biophysical Profile
Drosophila
Alzheimer's Disease

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