Photoinactivation of Mycobacteria in vitro and in a new murine model of localized Mycobacterium bovis BCG-induced granulomatous infection.

Antimicrobial Agents and Chemotherapy
Katie O'RiordanTayyaba Hasan

Abstract

Treatment of tuberculosis is currently hindered by prolonged antibiotic regimens and the emergence of significant drug resistance. Alternatives and adjuncts to standard antimycobacterial agents are needed. We propose that a direct attack utilizing photosensitizers and light-based treatments may be effective in curtailing Mycobacterium tuberculosis in discrete anatomical sites in the most infectious phase of pulmonary tuberculosis. To demonstrate experimental proof of principle, we have applied established photodynamic therapy (PDT) technology to in vitro cultures and an in vivo mouse model using Mycobacterium bovis BCG. We report here in vitro and in vivo PDT efficacy studies and the use of a three-dimensional collagen gel as a delivery vehicle for BCG, subcutaneously inserted, to induce specifically localized granuloma-like lesions in mice. When a benzoporphyrin derivative was utilized as the photosensitive agent, exposure to light killed extracellular and intracellular BCG in significant numbers. Collagen scaffolds containing BCG inserted in situ in BALB/c mice for 3 months mimicked granulomatous lesions and demonstrated a marked cellular infiltration upon histological examination, with evidence of caseating necrosis and fibr...Continue Reading

References

May 17, 2003·The Journal of Infectious Diseases·Michael R HamblinTayyaba Hasan
Jun 27, 2003·BMC Immunology·Christine A WellsDavid A Hume
Dec 31, 2003·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Tetsuya OkunakaYukari Kuroiwa
Jan 2, 2004·Nature Medicine·Hannah Hoag
Feb 28, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·John Chan, JoAnne Flynn
May 4, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Giulio Jori, Stanley B Brown
May 4, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Michael R Hamblin, Tayyaba Hasan
Sep 29, 2004·Current Opinion in Microbiology·Ken Duncan, Clifton E Barry
Mar 1, 2005·The Annals of Thoracic Surgery·Shin-ichi TakedaRyoji Maekura
Mar 16, 2005·The Lancet Infectious Diseases·C Fordham von Reyn
May 13, 2005·Photodermatology, Photoimmunology & Photomedicine·Kirsten Iversen MøllerHans Christian Wulf
May 27, 2005·Antimicrobial Agents and Chemotherapy·Tatiana N Demidova, Michael R Hamblin
Jul 14, 2005·Nature Reviews. Microbiology·Peter Andersen, T Mark Doherty
Oct 15, 2005·Clinical Microbiology Reviews·T Mark Doherty, Peter Andersen
Dec 1, 2004·Photodiagnosis and Photodynamic Therapy·Ana P CastanoMichael R Hamblin
Dec 1, 2005·Photodiagnosis and Photodynamic Therapy·Katie O'RiordanTayyaba Hasan

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Citations

Jul 7, 2009·Lasers in Medical Science·J LinT T Dong
Feb 14, 2013·Photomedicine and Laser Surgery·Akira HasebeKen-ichiro Shibata
Aug 8, 2009·The Journal of Antimicrobial Chemotherapy·Elke Feese, Reza A Ghiladi
Dec 6, 2008·Folia Histochemica Et Cytobiologica·Dominik StrapagielWiesława Rudnicka
Sep 26, 2013·Current Opinion in Pharmacology·Rui YinMichael R Hamblin
Nov 26, 2009·Photodiagnosis and Photodynamic Therapy·Tianhong DaiMichael R Hamblin
Nov 17, 2015·Journal of Medicinal Chemistry·José A González-DelgadoBruno Sarmento
Apr 25, 2015·Journal of Photochemistry and Photobiology. B, Biology·Ji-Eun ChangSanghoon Jheon
Apr 24, 2018·Giornale Italiano Di Dermatologia E Venereologia : Organo Ufficiale, Società Italiana Di Dermatologia E Sifilografia·Vanesa Pérez-LagunaYolanda Gilaberte
Oct 5, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Katie O'RiordanTayyaba Hasan
Sep 30, 2015·Bioscience Reports·Zenildo Santos SilvaMichael R Hamblin
Mar 30, 2020·Dermatologic Therapy·Alessia BarisaniSabina Vaccari
Jun 6, 2014·Chemical Communications : Chem Comm·Manpreet BhattiSalvador Tomas
Dec 15, 2018·International Endodontic Journal·G PlotinoM Mercade
Jul 13, 2018·Frontiers in Microbiology·Xiaoqing HuMichael R Hamblin
Nov 13, 2019·Applied Microbiology and Biotechnology·Margarita O ShleevaArseny S Kaprelyants
Sep 28, 2020·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Kevim Bordignon GuterresBernardo Almeida Iglesias
Nov 20, 2020·Photodiagnosis and Photodynamic Therapy·Sepehr TorabiReza Fekrazad
May 18, 2021·Applied Microbiology and Biotechnology·Margarita ShleevaArseny Kaprelyants
Aug 19, 2020·Microbial Pathogenesis·Grazielle Guidolin RossiMarli Matiko Anraku de Campos
May 2, 2019·ACS Central Science·Amit K DuttaClaudia Jessen-Trefzer
Oct 28, 2021·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Rafael T ArosoMariette M Pereira

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