PMID: 7023538Jul 20, 1981

Photoinactivation of the thiamine transport system in Saccharomyces cerevisiae with 4-azido-2-nitrobenzoylthiamine

Biochimica Et Biophysica Acta
K SempukuA Iwashima


A newly synthesized photoreactive thiamine derivative, 4-azido-2-nitrobenzoylthiamine was found to be a competitive inhibitor of the thiamine transport system in Saccharomyces cerevisiae, exhibiting an apparent Ki of 36 nM. When exposed to visible light, 4-azido-2-nitrobenzoylthiamine irreversibly inactivated the thiamine transport. 4-Azido-2-nitrobenzoylthiamine-dependent photoinactivation of thiamine transport was partially protected by thiamine, but not by the nitrene-trapping reagent p-aminobenzoate. On the other hand, the irradiation of the yeast cells in the presence of 4-azido-2-nitrobenzoylthiamine did not significantly lead to inactivation of the biotin transport system. The results suggest that 4-azido-2-nitrobenzoylthiamine is a specific irreversible inhibitor of the thiamine transport system in Saccharomyces cerevisiae.


Dec 1, 1975·Biochimica Et Biophysica Acta·A IwashimaY Nose
Nov 2, 1979·Biochimica Et Biophysica Acta·A IwashimaY Nose
Sep 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·A E RuohoS J Singer
Dec 13, 1973·Biochimica Et Biophysica Acta·A IwashimaY Nose

Related Concepts

Plasma Membrane
Saccharomyces cerevisiae
Thiamine Mononitrate

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