Photoinduced conformational changes in DNA by poly(vinyl alcohol) carrying a malachite green moiety for protecting DNA against attack by nuclease

Soft Matter
Ryoko M Uda, Takashi Matsui

Abstract

Light is a highly advantageous means of specific cell targeting. Though targeted gene delivery is an important characteristic of an ideal delivery vehicle, there has been little effort to develop a photoresponsive vector. Among nonviral vectors, cationic substances interact effectively with negatively charged DNA. With this property in mind, we designed copolymers of poly(vinyl alcohol) carrying a malachite green moiety (PVAMG) with different molecular weights. Though PVAMG has no affinity for DNA in the absence of light, it undergoes photoionization in the presence of light to afford cationic DNA binding sites. The DNA-PVAMG complex was investigated with respect to DNA conformational changes and its protective nature, which are important properties for nonviral vectors. PVAMG irradiation promoted DNA conformational transitions from coils to partial globules to compacted globules. The complex had a protective effect against DNase I after PVAMG irradiation, while DNA was degraded under dark conditions. The effect on DNA transition and the protective nature were sensitive to the molecular weight of PVAMG. The data regarding binding constants and binding mode provided insight into the structure of the DNA-PVAMG complex. To withsta...Continue Reading

References

Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P L FelgnerM Danielsen
Sep 29, 1981·Biochemistry·L P WakelinM J Waring
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·O BoussifJ P Behr
Nov 1, 1994·Bioconjugate Chemistry·J S RemyJ P Behr
Sep 1, 1993·Bioconjugate Chemistry·J Haensler, F C Szoka
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·C J WheelerS H Cheng
Nov 1, 1996·Bioconjugate Chemistry·M X TangF C Szoka
Jan 1, 1997·Journal of the National Cancer Institute·J A Roth, R J Cristiano
Sep 26, 1997·Nature·I M Verma, N Somia
Jun 8, 2000·Science·E Marshall
Sep 12, 2002·Biophysical Chemistry·Naoko Makita, Kenichi Yoshikawa
May 2, 2003·Nature Reviews. Cancer·Dennis E J G J DolmansRakesh K Jain
Dec 4, 2003·Journal of the American Chemical Society·Jeremy R BabendureRoger Y Tsien
May 14, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Yu-Chuan LiuC Ted Lee
Jul 20, 2011·The Journal of Physical Chemistry. B·E FroehlichH A Tajmir-Riahi
Jun 1, 2014·The Journal of Physical Chemistry. B·Debabrata DeyDibakar Dhara

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Citations

Aug 16, 2017·Chemical Communications : Chem Comm·Yoshiyuki NonoguchiTsuyoshi Kawai

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