Photoinduced electron transfer in the cytochrome c/cytochrome c oxidase complex using thiouredopyrenetrisulfonate-labeled cytochrome c. Optical multichannel detection

Biochemistry
I SzundiO Einarsdóttir

Abstract

Intramolecular electron transfer in the electrostatic cytochrome c oxidase/cytochrome c complex was investigated using a novel photoactivatable dye. Laser photolysis of thiouredopyrenetrisulfonate (TUPS), covalently linked to cysteine 102 on yeast iso-1-cytochrome c, generates a triplet state of the dye, which donates an electron to cytochrome c, followed by electron transfer to cytochrome c oxidase. Time-resolved optical absorption difference spectra were collected at delay times from 100 ns to 200 ms between 325 and 650 nm. On the basis of singular value decomposition (SVD) and multiexponential fitting, three apparent lifetimes were resolved. A sequential kinetic mechanism is proposed from which the microscopic rate constants and spectra of the intermediates were determined. The triplet state of TUPS donates an electron to cytochrome c with a forward rate constant of approximately 2.0 x 10(4) s(-1). A significant fraction of the triplet returns back to the ground state on a similar time scale. The reduction of cytochrome c is followed by faster electron transfer from cytochrome c to Cu(A), with the equilibrium favoring the reduced cytochrome c. Subsequently, Cu(A) equilibrates with heme a with an apparent rate constant of app...Continue Reading

References

Apr 1, 1975·The Biochemical Journal·M T WilsonE Antonini
Sep 6, 2000·European Journal of Biochemistry·A KotlyarO Einarsdóttir

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Citations

Aug 5, 2004·Photochemistry and Photobiology·Alexander B KotlyarLászló Zimányi
Jan 29, 2008·Biophysical Journal·Marcel G FriedrichRenate L C Naumann
Jun 20, 2006·Biochemical and Biophysical Research Communications·Vicente Rodríguez-RoldánMiguel A De la Rosa
Feb 27, 2008·Acta Biologica Hungarica·Katalin TengerG Rákhely
Apr 22, 2004·Biochimica Et Biophysica Acta·Bryan SchmidtShelagh Ferguson-Miller
Apr 22, 2004·Biochimica Et Biophysica Acta·Olöf Einarsdóttir, Istvan Szundi
Mar 23, 2011·Biochimica Et Biophysica Acta·M Fátima LucasVictor Guallar
Jun 23, 2006·Photochemistry and Photobiology·John S Winterle, Olöf Einarsdóttir
Aug 15, 2006·The Journal of Biological Chemistry·Daniel JancuraMarian Fabian
Aug 26, 2003·The Journal of Biological Chemistry·Oliver ManegFrancesco Malatesta
Mar 12, 2021·Biochemistry. Biokhimii︠a︡·Ludmila MikulovaMarian Fabian
May 11, 2021·Biochimica Et Biophysica Acta. Bioenergetics·T SztachovaM Fabian
Jun 15, 2018·Biochemistry·Katarina KopcovaMarian Fabian
Nov 29, 2005·Journal of Chemical Information and Modeling·Katalin TengerLászló Zimányi

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