Photoisomers: obfuscating factors in clinical peroxidase measurements of unbound bilirubin?

Pediatrics
A F McDonaghDavid K Stevenson

Abstract

The objectives of the study were to measure the effect of 4Z,15E-bilirubin on peroxidase free bilirubin measurements and to review the literature on this topic. 4Z,15E-Bilirubin was generated in situ in serum or serum albumin solution through controlled irradiation of isomerically pure 4Z,15Z-bilirubin IXalpha, under conditions in which the total amount of bilirubin remained constant. Reactions were monitored by difference spectroscopy, to ensure that solutions were not irradiated beyond the initial photostationary state and that concentrations of other isomers were kept to a minimum. Prepared in this way, 10% to 25% of the total bilirubin in the final solutions was in the form of the 4Z,15E-isomer. Free bilirubin in the solutions was measured with a peroxidase method, before and after irradiation. The use of bovine serum albumin as a surrogate for human albumin in in vitro studies also was investigated. The findings of previous studies are not altogether consistent, with a common flaw in several being the failure to measure photoisomer concentrations. For bilirubin in serum albumin solution, conversion of approximately 25% of the 4Z,15Z-isomer to 4Z,15E-bilirubin led to a much smaller decrease (<20%) in the apparent free bilir...Continue Reading

Citations

Nov 22, 2013·The New England Journal of Medicine·Jon F Watchko, Claudio Tiribelli
Jan 15, 2014·Analytica Chimica Acta·Mitja MartelancMladen Franko
Oct 12, 2010·Journal of Perinatology : Official Journal of the California Perinatal Association·V K BhutaniL Hamerman-Johnson
May 29, 2016·Clinics in Perinatology·Thor Willy Ruud Hansen
Jun 7, 2011·Seminars in Perinatology·Sanjiv B Amin, Angelo A Lamola
Jan 6, 2010·Journal of Photochemistry and Photobiology. B, Biology·Takashi IwaseSusumu Itoh
Nov 26, 2009·Seminars in Fetal & Neonatal Medicine·Antony F McDonagh
Jan 29, 2011·Acta Paediatrica·Susan R HintzUNKNOWN Eunice Kennedy Shriver National Institute of Child Health and Human Development Neonatal Research Network
May 29, 2016·Clinics in Perinatology·Sanjiv B Amin
Nov 30, 2014·Seminars in Fetal & Neonatal Medicine·Ichiro MoriokaHajime Nakamura
May 9, 2009·Clinical Chemistry·Charles E AhlforsClaudio Tiribelli
Mar 8, 2012·Clinical Chemistry·Andrew H HuberAlan M Kleinfeld
Mar 14, 2012·Clinical Chemistry·Richard P Wennberg
Feb 6, 2018·Luminescence : the Journal of Biological and Chemical Luminescence·Saikh M WabaidurZeid A Alothman
Oct 23, 2012·Annals of Clinical Biochemistry·Hitoshi OkadaSusumu Itoh
Aug 6, 2021·Laboratory Medicine·Tran Tien TaiShigeru Taketani

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.