Photolysis of caged inositol 1,4,5-trisphosphate induces action potentials in frog vomeronasal microvillar receptor neurones

Neuroscience
J GjerstadK B Døving

Abstract

To study the effect of inositol 1,4,5-trisphosphate (IP(3)) in isolated frog vomeronasal microvillar receptor neurones, whole-cell recordings were performed with 0.5 microM caged IP(3) dissolved in the pipette solution. IP(3) was released by photolysis of caged IP(3) initiated by a 0.8-ms ultraviolet flash from a xenon flash lamp 70 s after the start of dialysis of caged IP(3) into the cell. Flash illuminating the whole receptor neurone with caged IP(3) triggered action potentials when the current was clamped at zero and a series of transient inward currents of 12-55 pA at a holding potential of -70 mV. The average number of spikes during the first 40 s after release of IP(3) was 7.2+/-2.5 (n=6, mean+/-S.E.M.). The average maximum current and the total inward transport of charge during the first 40 s after photolysis of caged IP(3) were -24+/-8.0 pA and -1.7+/-0.8 pC, respectively (n=5, mean+/-S.E.M.). Inward membrane currents of 12-55 pA after release of IP(3) were not observed with 50 microM La(3+) in the bath. Notably, flash focused on the terminal vesicle also triggered action potentials. No action potentials were observed following flash focused on the soma or outside the dendrite. The average number of spikes during the f...Continue Reading

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Citations

Jul 20, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Wendy E Grus, Jianzhi Zhang
Jul 11, 2006·Angewandte Chemie·Günter Mayer, Alexander Heckel

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