Photometric and fluorometric alkaline phosphatase assays using the simplest enzyme substrates

Talanta
Justyna BzuraRobert Koncki

Abstract

In this contribution a highly cost-effective flow analysis system for determination of alkaline phosphatase (ALP) activity is presented. This fully-mechanized bioanalytical system is based on economic solenoid micropumps and microvalves (powered and actuated by Arduino microcontroller) and extremely cheap dedicated optoelectronic flow-through detectors allowing absorbance and fluorescence measurements. The detection schemes for ALP assaying realized in this system are based on orthophosphate determination. For the detection of these ions formed in the course of enzymatic conversion a molybdate method requiring only common and inexpensive chemicals is utilized. Thus, for the enzymatic assays the simplest not-chromogenic/not-fluorogenic ALP substrates can be applied. Such approach results in the use of low-cost reagents for ALP assays, whereas the mechanization of assay causes low consumption of reagents as well as samples. In the course of reported investigations six ALP substrates were examined and the most promising results have been obtained for the inorganic compound - monofluorophosphate (MFP). The obtained linear ranges of absorbance and fluorescence measurements are 100-600 U L-1 and 30-30/30-100 U L-1, with sensitivities...Continue Reading

References

Sep 30, 1994·Journal of Biotechnology·M Sánchez-CabezudoM D Luque de Castro
Nov 25, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Xiaojing Zhu, Chongqiu Jiang
Aug 29, 2007·Analytica Chimica Acta·Robert KonckiŁukasz Tymecki
Jul 24, 2012·Talanta·Kamil StrzelakLukasz Tymecki
Jul 26, 2014·Talanta·Marta FiedorukLukasz Tymecki
Jan 16, 2015·Biosensors & Bioelectronics·C Lorena Manzanares-PalenzuelaBeatriz López-Ruiz
Jun 17, 2015·Talanta·Pablo GonzálezElias A G Zagatto
May 3, 2016·Journal of Pharmaceutical and Biomedical Analysis·Michał MichalecRobert Koncki
Sep 4, 2016·Talanta·Marta PokrzywnickaŁukasz Tymecki
Jan 7, 2018·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Akif Goktug BozkurtIsmail Hakki Boyaci

❮ Previous
Next ❯

Citations

Apr 2, 2020·Environmental Science and Pollution Research International·Seyed Mohammad HosseiniNegin Siahchehreh Badeli
May 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thitirat MantimDuangjai Nacapricha

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.