Photomodification of the electrical properties of the plasma membrane: a comparison between 6 different membrane-active photosensitizers

The Journal of Membrane Biology
F KilligG Stark

Abstract

The present study deals with photomodification of the electrical properties of the plasma membrane of an epithelial cell line (opossum kidney (OK) cells). The effect of photofrin II (previously investigated) is compared with that of 5 other membrane-active sensitizers: sulfonated Zn-phthalocyanine, merocyanine 540, rose bengal, methylene blue and protoporphyrin IX (an endogenous sensitizer induced by addition of its biosynthetic precursor 5-aminolaevulinic acid). The study was performed in order to investigate whether photomodification of the ion transport properties of the plasma membrane by membrane-active sensitizers is a general and early event in cellular photosensitization. The changes in the electrical properties were monitored by application of the whole-cell and the inside-out configuration of the patch-clamp technique. Illumination in the presence of the compounds (apart from merocyanine 540) gave rise to similar changes of the electrical properties of the membrane: depolarization of the membrane potential, inactivation of a large-conductance, Ca2+-dependent K+-channel (maxi-KCa), and a strong increase of the leak conductance of the membrane. This similarity indicates the general character of the functional photomodif...Continue Reading

Citations

May 1, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Takeshi KatsuragiKeisuke Migita
Feb 6, 2010·Naunyn-Schmiedeberg's Archives of Pharmacology·Yumei ZhaoTakeshi Katsuragi
Sep 1, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Sandrine DulongJordi Ehrenfeld
Jul 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Gregory ArcuinoMaiken Nedergaard
Jun 2, 2010·Biochemical and Biophysical Research Communications·Jing SunTakeshi Katsuragi
Jun 11, 2002·Biochemical and Biophysical Research Communications·Takeshi KatsuragiKenji Honda
Jun 21, 2005·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Nora NaumannAngela Rösen-Wolff
Sep 11, 2007·Journal of Pharmacological Sciences·Yumei ZhaoTakeshi Katsuragi
Sep 17, 2004·American Journal of Physiology. Cell Physiology·David Reigada, Claire H Mitchell
Oct 26, 2005·The Journal of Membrane Biology·G Stark
Jan 4, 2012·Photochemistry and Photobiology·Hisato KatoTakashi Katsu
Mar 4, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Alfonso Blázquez-CastroAngeles Juarranz
Aug 30, 2005·Biochimica Et Biophysica Acta·Hérica de Lima SantosPietro Ciancaglini

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