Photomodulating Gene Expression by Using Caged siRNAs with Single-Aptamer Modification

Chembiochem : a European Journal of Chemical Biology
Liangliang ZhangXinjing Tang

Abstract

Caged siRNAs incorporating terminal modification were rationally designed for photochemical regulation of gene silencing induced by RNA interference (RNAi). Through the conjugation of a single oligonucleotide aptamer at the 5' terminus of the antisense RNA strand, enhancement of the blocking effect for RNA-induced silencing complex (RISC) formation/processing was expected, due both/either to the aptamers themselves and/or to their interaction with large binding proteins. Two oligonucleotide aptamers (AS1411 and MUC-1) were chosen for aptamer-siRNA conjugation through a photolabile linker. This caging strategy was successfully used to photoregulate gene expression both of firefly luciferase and of green fluorescent protein (GFP) in cells. Further patterning experiments revealed that spatial regulation of GFP expression was successfully achieved by using the aptamer-modified caged siRNA and light activation. We expect that further optimized caged siRNAs featuring aptamer conjugation will be promising for practical applications to spatiotemporal photoregulation of gene expression in the future.

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Citations

Jul 25, 2018·Critical Reviews in Biochemistry and Molecular Biology·Robert M Hughes
Jul 14, 2020·Biochemical Society Transactions·Denis HartmannMichael J Booth
Jul 18, 2020·Chemistry : a European Journal·Jinhao ZhangXinJing Tang
Jul 2, 2019·International Journal of Pharmaceutics·Marimuthu CitartanThean-Hock Tang
Jan 13, 2021·Chembiochem : a European Journal of Chemical Biology·Qian WangXinjing Tang
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Linlin Yang, Ivan J Dmochowski
Sep 18, 2021·Organic & Biomolecular Chemistry·Jun-Ichi Mihara, Kenzo Fujimoto

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