Photonic crystal enhanced fluorescence immunoassay on diatom biosilica

Journal of Biophotonics
Kenneth SquireAlan X Wang

Abstract

Fluorescence biosensing is one of the most established biosensing methods, particularly fluorescence spectroscopy and microscopy. These are two highly sensitive techniques but require high-grade electronics and optics to achieve the desired sensitivity. Efforts have been made to implement these methods using consumer grade electronics and simple optical setups for applications such as point-of-care diagnostics, but the sensitivity inherently suffers. Sensing substrates, capable of enhancing fluorescence are thus needed to achieve high sensitivity for such applications. In this paper, we demonstrate a photonic crystal-enhanced fluorescence immunoassay biosensor using diatom biosilica, which consists of silica frustules with sub-100 nm periodic pores. Utilizing the enhanced local optical field, the Purcell effect and increased surface area from the diatom photonic crystals, we create ultrasensitive immunoassay biosensors that can significantly enhance fluorescence spectroscopy as well as fluorescence imaging. Using standard antibody-antigen-labeled antibody immunoassay protocol, we experimentally achieved 100× and 10× better detection limit with fluorescence spectroscopy and fluorescence imaging respectively. The limit of detecti...Continue Reading

References

Apr 28, 2005·Biosensors & Bioelectronics·John C PickupDavid J S Birch
Sep 1, 2005·Journal of Agricultural and Food Chemistry·Ewa SikorskaMarek Sikorski
Jul 26, 2008·Nature Nanotechnology·Nikhil GaneshBrian T Cunningham
Oct 30, 2006·Optics Express·Yu-Ju HungHsuan-Chen Wu
Jul 23, 2009·PloS One·David N BreslauerDaniel A Fletcher
Nov 11, 2010·Lab on a Chip·Hongying ZhuAydogan Ozcan
Dec 18, 2010·Optics Express·Anusha PokhriyalBrian T Cunningham
May 23, 2012·Chemical Communications : Chem Comm·Fang LuoGuonan Chen
Apr 24, 2013·Lab on a Chip·Dustin GallegosBrian T Cunningham
Sep 3, 2013·Lab on a Chip·Ahmet F CoskunAydogan Ozcan
Mar 1, 2014·Nature Methods·Hendrik DeschoutKevin Braeckmans
Aug 8, 2014·Analytical Chemistry·Hojeong YuBrian T Cunningham
Dec 31, 2014·Nature Methods·Ernst H K Stelzer
Jun 10, 2016·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Anjali V SheahanTarik F Massoud
Jan 1, 2014·Plasmonics·Martin BauchJakub Dostalek
Nov 11, 2016·Biosensors & Bioelectronics·Xun LuJ Justin Gooding

❮ Previous
Next ❯

Citations

Dec 5, 2019·Sensors·Ilaria Rea, Luca De Stefano
Jan 3, 2021·Luminescence : the Journal of Biological and Chemical Luminescence·Georgina González-FortunaJ Viridiana García-Meza
Jun 22, 2021·ACS Biomaterials Science & Engineering·Navid RabieeRajender S Varma

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.