Photonic Crystal Optical Tweezers with High Efficiency for Live Biological Samples and Viability Characterization

Scientific Reports
Peifeng JingLih Y Lin

Abstract

We propose and demonstrate a new optical trapping method for single cells that utilizes modulated light fields to trap a wide array of cell types, including mammalian, yeast, and Escherichia coli cells, on the surface of a two-dimensional photonic crystal. This method is capable of reducing the required light intensity, and thus minimizing the photothermal damage to living cells, thereby extending cell viability in optical trapping and cell manipulation applications. To this end, a thorough characterization of cell viability in optical trapping environments was performed. This study also demonstrates the technique using spatial light modulation in patterned manipulation of live cell arrays over a broad area.

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Citations

Mar 14, 2017·Applied Spectroscopy·Donato ConteducaCaterina Ciminelli
Feb 9, 2017·Microscopy Research and Technique·Kavya Mohan, Partha Pratim Mondal
Oct 19, 2016·Lab on a Chip·Roozbeh Abedini-NassabBenjamin B Yellen
Sep 15, 2017·Stem Cells·Yong Kyun KimBenjamin S Freedman
Sep 30, 2020·Expert Opinion on Drug Discovery·Carley OrtChristopher Moraes
Nov 8, 2018·Micromachines·Adrian Martinez-RivasEtienne Dague
Jan 16, 2019·Biomolecules·Dhawal ChoudharyCiro Cecconi
Jul 2, 2018·The Review of Scientific Instruments·Kavya MohanPartha Pratim Mondal
Aug 3, 2019·Micromachines·Alfonso Blázquez-Castro
Mar 20, 2018·Biomedical Optics Express·Peifeng JingLih Y Lin
Mar 27, 2021·The Journal of the Acoustical Society of America·Björn HammarströmMartin Wiklund
Mar 2, 2021·Annual Review of Biophysics·Souradeep BanerjeeShubhasis Haldar
Feb 16, 2019·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Xiaolei PengYuebing Zheng
Mar 27, 2017··Yu-Chia ChenBenjamin S. Freedman
May 4, 2018··Giovanni MagnoGiovanna Calò

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Methods Mentioned

BETA
optical tweezers

Software Mentioned

MEEP
Tracker

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