Photonic crystal protein hydrogel sensor materials enabled by conformationally induced volume phase transition

Chemical Science
Zhongyu CaiSanford A Asher

Abstract

Hydrogels that change volume in response to specific molecular stimuli can serve as platforms for sensors, actuators and drug delivery devices. There is great interest in designing intelligent hydrogels for tissue engineering, drug delivery, and microfluidics that utilize protein binding specificities and conformational changes. Protein conformational change induced by ligand binding can cause volume phase transitions (VPTs). Here, we develop a highly selective glucose sensing protein photonic crystal (PC) hydrogel that is fabricated from genetically engineered E. coli glucose/galactose binding protein (GGBP). The resulting 2-D PC-GGBP hydrogel undergoes a VPT in response to glucose. The volume change causes the 2-D PC array particle spacing to decrease, leading to a blue-shifted diffraction which enables our sensors to report on glucose concentrations. This 2-D PC-GGBP responsive hydrogel functions as a selective and sensitive sensor that easily monitors glucose concentrations from ∼0.2 μM to ∼10 mM. This work demonstrates a proof-of-concept for developing responsive, "smart" protein hydrogel materials with VPTs that utilize ligand binding induced protein conformational changes. This innovation may enable the development of ot...Continue Reading

References

Jul 3, 2003·Nature Reviews. Drug Discovery·Simon J Teague
Oct 2, 2004·Clinical Chemistry·Vladimir L AlexeevSanford A Asher
Mar 15, 2005·Nature Materials·Jason D EhrickSylvia Daunert
Nov 18, 2005·Journal of Colloid and Interface Science·Chad E Reese, Sanford A Asher
Mar 17, 2007·Angewandte Chemie·William L MurphyMilan Mrksich
May 3, 2007·Protein Science : a Publication of the Protein Society·M Jack BorrokKatrina T Forest
Jan 1, 1984·Biotechnology and Bioengineering·H TanakaI A Veliky
Nov 5, 2008·Journal of the American Chemical Society·Weiwei YuanJindrich Kopecek
May 13, 2009·Macromolecular Bioscience·Jason D EhrickSylvia Daunert
May 9, 2012·Angewandte Chemie·Jian-Tao ZhangSanford A Asher
Nov 15, 2012·Journal of the American Chemical Society·Gabriel OrtegaOscar Millet
Jun 8, 2013·Chemical Communications : Chem Comm·Jian-Tao ZhangSanford A Asher
Apr 29, 2014·Analytical Chemistry·Zhongyu CaiSanford A Asher
Aug 28, 2014·Analytical Chemistry·Jian-Tao ZhangSanford A Asher
Apr 14, 2015·Analytical Chemistry·Zhongyu CaiSanford A Asher
Oct 21, 2015·Angewandte Chemie·Zhongyu CaiSanford A Asher

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Citations

Nov 6, 2018·Chemical Communications : Chem Comm·Yun ChenYi Chen
Jan 24, 2020·The Analyst·Chen Chen, Junsheng Wang
Mar 19, 2020·Polymers·Wenwei Tang, Cheng Chen
Jan 24, 2018·Small·Ting WangXiaodong Chen
Feb 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Tengfei WangFuqiang Nie
Nov 11, 2017·Chemical Communications : Chem Comm·Zelinlan Wang, Zhiguang Guo
Dec 11, 2019·ACS Applied Materials & Interfaces·Natasha Lynn SmithSanford A Asher
Mar 30, 2021·Frontiers in Chemistry·Jingying WangQiang Zhang
Sep 30, 2016·ACS Applied Materials & Interfaces·Qiang Matthew ZhangMichael J Serpe
Jun 16, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Takuma Kureha, Daisuke Suzuki
Sep 12, 2019·ACS Nano·Yixiao DongKhalid Salaita
Aug 26, 2021·Chemistry : a European Journal·Kiefer O RambergPeter B Crowley
Nov 27, 2021·Advanced Science·Yuntao LiuGuoqing Pan

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Methods Mentioned

BETA
irradiating
X-ray
electrophoresis
NMR

Software Mentioned

MATLAB
MSP

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