Jan 11, 2013

Photooxidation of glycated and non-glycated phosphatidylethanolamines monitored by mass spectrometry

Journal of Mass Spectrometry : JMS
Tânia MeloM Rosário M Domingues

Abstract

Phosphatidylethanolamines (PE) are one of the major components of cells membranes, namely in skin and in retina, that are continuously exposed to solar UV radiation being major targets of photooxidation damage. In addition, due to the presence of the free amine group, PE can also undergo glycation, in hyperglycemic conditions which may increase the susceptibility to oxidation. The aim of this study is to develop a model, based on mass spectrometry (MS) analysis, to identify photooxidative degradation of selected PE (POPE: PE 16:0/18:1, PLPE: PE 16:0/18:2, PAPE: PE 16:0/20:4) and glycated PEs due to UV irradiation. Photooxidation products were analysed by electrospray ionization MS (ESI-MS) and tandem MS (ESI-MS/MS) in positive and negative mode. Emphasis is placed in the influence of glycation in the generation of distinct photooxidation products. ESI-MS spectra of PE after UV photo-irradiation showed mainly hydroperoxy derivatives, due to oxidation of unsaturated fatty acyl chains. Glycated PE gave rise to several new photooxidation products formed due to oxidative cleavages of the glucose moiety, namely between C1 and C2, C2 and C3, and C5 and C6 of this sugar unit. These new products were identified by ESI-MS/MS in positive ...Continue Reading

  • References44
  • Citations8

References

  • References44
  • Citations8

Citations

Mentioned in this Paper

Tissue Membrane
Derivatives
Entire Retina
Complement Component 2 Deficiency
Lipid Peroxides
Retinal Diseases
Tandem Mass Spectrometry
Etiology
Microvascular Complications OF Diabetes, Susceptibility TO, 1(Finding)
Oxidation

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