PMID: 2119804Aug 7, 1990Paper

Photophysics of metalloazurins

Biochemistry
J E HansenG R Fleming

Abstract

The fluorescence lifetimes of Cu(II), Cu(I), Ag(I), Hg(II), Co(II), and Ni(II) azurin Pae from Pseudomonas aeruginosa and Cu(II), Cu(I), and Hg(II) azurin Afe from Alcaligenes faecalis were measured at 295 K by time-correlated single-photon counting. In addition, fluorescence lifetimes of Cu(II) azurin Pae were measured between 30 and 160 K and showed little change in value. Ultraviolet absorption difference spectra between metalloazurin Pae and apoazurin Pae were measured, as were the fluorescence spectra of metalloazurins. These spectra were used to determine the spectral overlap integral required for dipole-dipole resonance calculations. All metalloazurins exhibit a reduced fluorescence lifetime compared to their respective apoazurins. Forster electronic energy transfer rates were calculated for both metalloazurin Pae and metalloazurin Afe derivatives; both enzymes contain a single tryptophyl residue which is located in a different position in the two azurins. These azurins have markedly different fluorescence spectra, and electronic energy transfers occur from these two tryptophyl sites with different distances and orientations and spectral overlap integral values. Intramolecular distances and orientations were derived from...Continue Reading

References

Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D R McMillinH B Gray
Apr 2, 1973·European Journal of Biochemistry·A Finazzi-AgròB Mondovì
Jan 1, 1974·CRC Critical Reviews in Biochemistry·E H Strickland
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Citations

Oct 17, 1998·Biochimica Et Biophysica Acta·M Hof
Jun 12, 2010·Journal of the American Chemical Society·Hannah S ShafaatJudy E Kim
Oct 3, 1999·European Journal of Biochemistry·G MeiA Finazzi-Agrò
Feb 16, 2006·IEE Proceedings. Nanobiotechnology·R RinaldiR Cingolani
May 28, 2005·The Journal of Chemical Physics·P P PompaR Rinaldi
Nov 20, 2004·The Journal of Chemical Physics·P P PompaG W Canters
Jun 1, 2007·Angewandte Chemie·Jaap BroosPatrik R Callis
Apr 7, 2011·Chemistry : a European Journal·Ana María Blanco-RodríguezAntonín Vlček
Jul 5, 2008·Biophysical Journal·Karina Tveen JensenJ Baz Jackson
Jan 28, 2015·The Journal of Physical Chemistry. B·Bethany C LarsonJudy E Kim
Apr 20, 2004·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·P P PompaG W Canters
Apr 24, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Khrustalev Vladislav VictorovichAkunevich Anastasia Aleksandrovna
Jun 18, 2021·Inorganic Chemistry·Debanjana Das, Sri Rama Koti Ainavarapu
Jun 30, 2018·Inorganic Chemistry·Biplab K MaitiJosé J G Moura

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