Photopolymerization of polyacrylamide gels with methylene blue

Electrophoresis
T LyubimovaThierry Rabilloud

Abstract

Photopolymerization of polyacrylamide gels in the presence of methylene blue (100 microM) and a redox couple (1 mM sodium toluenesulfinate, a reducer, and 50 microM diphenyliodonium chloride, an oxidizer) has been investigated. The gel point, i.e. the time needed for onset of gelation upon illumination, has been found to lengthen progressively at lower temperatures and at lower light intensities. If the three catalysts are progressively diluted, the gel point does not vary for a threefold dilution, but gelation is greatly hampered below a 1:5 dilution of the three effectors. Photobleaching has been assessed as a function of liquid layer thickness (from 0.5 to 2 mm), of a progressive dye dilution (down to a fourfold dilution) and as a function of temperature. A maximum of elastic modulus is located in correspondence to a minimum of permeability (both situated at 5% cross-linker). It is found that methylene blue-activated polymerization produces polyacrylamide gels with elastic properties which are higher than in persulfate-activated gels, so far the most popular matrices for electrokinetic separations. Due to the ease of preparation, the full control of all experimental parameters, and the lack of oxidizing power of this catalys...Continue Reading

Citations

Mar 1, 1993·Electrophoresis·T Lyubimova, P G Righetti
Aug 1, 1994·Electrophoresis·P G RighettiV A Briskman
Oct 1, 1995·Electrophoresis·M Chiari, P G Righetti
May 1, 1996·Electrophoresis·T Rabilloud
Dec 10, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·P G Righetti, C Gelfi
May 4, 2011·Molecular and Cellular Biology·Dmitry E AgafonovReinhard Lührmann
Nov 16, 2010·Dental Materials : Official Publication of the Academy of Dental Materials·Taweesak PrasansuttipornJunji Tagami
Jun 12, 2009·Electrophoresis·Thierry Rabilloud
Dec 17, 2008·Mass Spectrometry Reviews·Thierry RabilloudMireille Chevallet
Jan 11, 2012·Analytical Biochemistry·Christopher C Saunders, Wesley E Stites
May 3, 2014·Restorative Dentistry & Endodontics·Maryam Khoroushi, Marzieh Kachuei
Apr 23, 2008·Current Protocols in Protein Science·D P Goldenberg
Nov 2, 2007·Chemical Reviews·Karel Kleparník, Petr Bocek
Aug 1, 1997·Analytical Biochemistry·D GuschinA Mirzabekov

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