Photoprotective effect of isoflavone genistein on ultraviolet B-induced pyrimidine dimer formation and PCNA expression in human reconstituted skin and its implications in dermatology and prevention of cutaneous carcinogenesis

Carcinogenesis
Julian O MooreHuachen Wei

Abstract

Genistein, the most abundant isoflavone of the soy derived phytoestrogen compounds, is a potent antioxidant and inhibitor of tyrosine kinase. We previously reported the antiphotocarcinogenic effects of genistein in SKH-1 murine skin, including its capacity for scavenging reactive oxygen species, inhibiting photodynamic DNA damage and downregulating UVB(ultra violet B)-induced signal transduction cascades in carcinogenesis. In this study we elucidate genistein's photoprotective efficacy within the context of full thickness human reconstituted skin relative to acute challenges with ultraviolet-B irradiation. Skin samples were pre-treated with three concentrations of genistein (10, 20 and 50 microM) 1 h prior to UVB radiation at 20 and 60 mJ/cm2. Proliferating cell nuclear antigen (PCNA) and pyrimidine dimer (PD) expression profiles were localized using immunohistochemical analysis on paraffin embedded samples 6 and 12 h post UVB exposure. Genistein dose dependently preserved cutaneous proliferation and repair mechanics at 20 and 60 mJ/cm2, as evidenced by the preservation of proliferating cell populations with increasing genistein concentrations and noticeable paucity in PCNA immunoreactivity in the absence of genistein. Genistei...Continue Reading

References

Jan 1, 1995·Proceedings of the Society for Experimental Biology and Medicine·H WeiY Wang
Jul 1, 1997·Journal of Pharmaceutical and Biomedical Analysis·M T KnorstW Wohlrab
Feb 1, 1997·Photodermatology, Photoimmunology & Photomedicine·C AugustinO Damour
Aug 15, 1998·Archives of Biochemistry and Biophysics·A AroraG M Strasburg
Jan 28, 1999·Biochemical and Biophysical Research Communications·J F ZhaoD R Bickers
Apr 14, 1999·Molecular Medicine Today·H van Steeg, K H Kraemer
Aug 4, 1999·Obstetrics and Gynecology·P AlbertazziM S Morton
Dec 14, 1999·The Journal of Investigative Dermatology·J F ZhaoZ Y Wang
Jun 7, 2000·Journal of the American College of Cardiology·G A FigtreeP Collins
Apr 4, 2001·The Journal of Nutrition·D Goodman-Gruen, D Kritz-Silverstein
Nov 17, 2001·The Journal of Investigative Dermatology·T R BertonS M Fischer
Mar 7, 2002·The Journal of Nutrition·Thomas B Clarkson
Mar 11, 2003·Climacteric : the Journal of the International Menopause Society·P AlbertazziM Bottazzi
Oct 31, 2003·Expert Reviews in Molecular Medicine·Yasuhiro Matsumura, Honnavara N Ananthaswamy
Nov 11, 2003·The Journal of Nutrition·Huachen WeiMark G Lebwohl
Nov 26, 2003·Journal of the American Academy of Dermatology·Michael R Albert, Kristen G Ostheimer
Jan 9, 2004·Cell Cycle·Rati FotedarArun Fotedar
Feb 21, 2004·CA: a Cancer Journal for Clinicians·Ahmedin JemalUNKNOWN American Cancer Society
Jun 12, 2004·The Journal of Investigative Dermatology·Fumikazu YamazakiTakeshi Horio
Dec 1, 2004·The Journal of Biological Chemistry·Markus BöhmAgatha Schwarz
Dec 2, 2004·Expert Opinion on Pharmacotherapy·Heather Boon, Jacqueline Wong
Dec 9, 2004·Advances in Experimental Medicine and Biology·Mepur H RavindranathSubramanian Viswanathan
Dec 9, 2004·Current Opinion in Urology·Jeffrey M HolzbeierleinJ Brantley Thrasher
Dec 30, 2004·Photochemistry and Photobiology·Julian O MooreHuachen Wei
Jan 29, 2005·International Journal of Cardiology·Angelo A IzzoEdzard Ernst
Feb 9, 2005·The American Journal of Clinical Nutrition·Siyan Zhan, Suzanne C Ho
Mar 8, 2005·Mutation Research·Daniel B YaroshDavid A Brown
Jul 8, 2005·Cancer Chemotherapy and Pharmacology·Richard D VerschoyleAndreas J Gescher
Oct 4, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R Michael McClainJochen Bausch
Aug 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C L CannonM Klausner

❮ Previous
Next ❯

Citations

Nov 10, 2009·Archives of Dermatological Research·Joi A Nichols, Santosh K Katiyar
Dec 15, 2010·Archives of Biochemistry and Biophysics·Farrukh Afaq
Jul 31, 2013·Journal of Photochemistry and Photobiology. B, Biology·Julie A Evans-JohnsonC-Y Oliver Chen
Dec 25, 2007·Antioxidants & Redox Signaling·Naghma KhanHasan Mukhtar
Apr 27, 2012·Rejuvenation Research·C C Zouboulis, E Makrantonaki
Aug 24, 2012·Journal of Radiation Research·Reem YunisDavid M Rocke
Feb 13, 2008·Photochemistry and Photobiology·Vaqar M AdhamiFarrukh Afaq
Aug 14, 2009·The Journal of Investigative Dermatology. Symposium Proceedings·Mary S MatsuiElma Baron
Jul 26, 2011·Journal of Biomedicine & Biotechnology·Barbara IovineMaria Assunta Bevilacqua
Mar 8, 2013·Dermato-endocrinology·Silke K SchagenChristos C Zouboulis
Feb 11, 2015·Journal of Photochemistry and Photobiology. B, Biology·V A TerraA L Cecchini
May 13, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Lígia Marquez AndradeStephânia Fleury Taveira
Jul 22, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Patrick J HaydenMitchell Klausner
Jan 8, 2017·Photochemistry and Photobiology·Mary K Montes de OcaFarrukh Afaq
Mar 23, 2018·International Journal of Molecular Sciences·Chau Yee NgShih-Chi Su
Sep 13, 2017·Oxidative Medicine and Cellular Longevity·Ana Ortiz-EspínFrancisca Sevilla
Aug 3, 2006·Experimental Dermatology·Farrukh Afaq, Hasan Mukhtar
May 5, 2011·Seminars in Cutaneous Medicine and Surgery·William L CampCraig A Elmets
Oct 22, 2011·Climacteric : the Journal of the International Menopause Society·Debora Yankelevich
Feb 3, 2012·Journal of Chromatographic Science·Bethânia Andrade de VargasHelder Ferreira Teixeira
Jul 17, 2013·Photochemistry and Photobiology·Anant AgrawalDianne E Godar
Jun 27, 2014·Food & Function·Elisabet Fernández-García
Mar 29, 2011·Critical Reviews in Toxicology·Susanne AndresAlfonso Lampen
Jul 1, 2020·International Journal of Molecular Sciences·Ekaterina ProshkinaAlexey Moskalev
Jul 1, 2011·American Journal of Clinical Dermatology·Yu Yu Shu, Howard I Maibach
Jan 30, 2020·International Journal of Molecular Sciences·Mengbing ChenYing Huang
Jun 10, 2014·Journal of Cosmetic Dermatology·Charlene DeHavenJonathan Oldach
Feb 22, 2011·International Journal of Dermatology·James Leyden, Warren Wallo
Sep 12, 2018·Journal of Cosmetic Dermatology·Sylvie BoisnicViolaine Jabbour
Dec 18, 2020·Frontiers in Oncology·Francisco Alejandro Lagunas-Rangel, Rosa María Bermúdez-Cruz
Jan 16, 2021·Nutrients·Monika MichalakEdyta Suliga
Mar 24, 2021·Chemical Research in Toxicology·Hsun-Hao ChangChi-Feng Hung
Jul 4, 2021·Progress in Biophysics and Molecular Biology·Tianmei LiRongji Dai
Dec 15, 2010·Clinics in Dermatology·Christos C Zouboulis, Evgenia Makrantonaki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.