PMID: 9188275Jun 1, 1997Paper

Photoreactivating enzyme for (6-4) photoproducts in cultured goldfish cells

Photochemistry and Photobiology
N UchidaA Shima

Abstract

We previously reported that when cultured goldfish cells are illuminated with fluorescent light, photorepair ability for both cyclobutane pyrimidine dimers and (6-4) photoproducts increased. In the present study, it was found that the duration of the induced photorepair ability for cyclobutane pyrimidine dimers was longer than that for (6-4) photoproducts, suggesting the presence of different photolyases for repair of these two major forms of DNA damage. A gel shift assay was then performed to show the presence of protein(s) binding to (6-4) photoproducts and its dissociation from (6-4) photoproducts under fluorescent light illumination. In addition, at 8 h after fluorescent light illumination of the cell, the binding of protein(s) to (6-4) photoproducts increased. The restriction enzymes that have recognition sites containing TT or TC sequences failed to digest the UV-irradiated DNA photoreactivated by using Escherichia coli photolyase for cyclobutane pyrimidine dimers, indicating that restriction enzymes could not function because (6-4) photoproducts remained in recognition sites. But, when UV-irradiated DNA depleted of cyclobutane pyrimidine dimers was incubated with extract of cultured goldfish cells under fluorescent light...Continue Reading

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Jun 1, 1989·Photochemistry and Photobiology·D L Mitchell, R S Nairn
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May 1, 1993·Photochemistry and Photobiology·S T Kim, A Sancar

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Citations

Aug 25, 2007·Journal of Photochemistry and Photobiology. B, Biology·Qiaoxiang DongW Todd Monroe
Sep 4, 2015·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Ronald B WalterMarkita G Savage
Jan 21, 2015·Genome Biology and Evolution·Marion F HaugStephan C F Neuhauss
Dec 20, 2016·Photochemistry and Photobiology·Junpei YamamotoKlaus Brettel
Aug 18, 2000·Photochemistry and Photobiology·J A MeadorD L Mitchell
Jan 26, 2008·Animal Health Research Reviews·S M DaboA W Confer
Aug 1, 1998·The Journal of Biological Chemistry·B J Vande Berg, G B Sancar
Jun 12, 2004·Photochemistry and Photobiology·David L MitchellRonald B Walter

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