Photoreceptor recovery in retinoid-deprived rats after vitamin A replenishment

Experimental Eye Research
M L KatzW S Stark

Abstract

Dietary deficiency in the retinoid precursors of the visual pigment chromophore 11-cis retinal results in the synthesis of photoreceptor outer segments containing opsin in excess of the vitamin A available for rhodopsin regeneration. This suggests that vitamin A-free opsin may be incorporated into newly synthesized outer segment disc membranes. If this opsin is functionally intact, it should be possible convert it to rhodopsin in vivo by providing the appropriate retinoids, and the resulting rhodopsin should should be able to mediate visual transduction. Experiments were conducted to evaluate this possibility and to identify the rate-limiting steps in photoreceptor recovery from retinoid depletion. Rates were maintained on diets either containing or lacking retinoid precursors of 11-cis retinal for 23 weeks, at which time outer segment opsin content greatly exceeded the availability of visual cycle retinoids in the retina. The retinoid-deprived animals were then each given a single intramuscular injection of all-trans retinol. At various time intervals after retinol administration, electroretinograms (ERGs) were recorded on some rats, and retinal rhodopsin contents were determined in others. At similar time intervals, blood and...Continue Reading

Citations

Sep 1, 1995·Nature Genetics·S G JacobsonE M Stone
Jan 23, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G I LiouW S Stark
Jul 25, 2017·The American Journal of Pathology·Volha V MalechkaJian-Xing Ma
Mar 29, 2021·Developmental Biology·Deepshe DewettJens Rister
Sep 29, 1995·Mechanisms of Ageing and Development·M L Katz, C L Gao

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